Recombinant

Recombinant Anti-LAG-3 antibody [CAL77] (ab237720)

Overview

  • Product name

    Anti-LAG-3 antibody [CAL77]
    See all LAG-3 primary antibodies
  • Description

    Rabbit monoclonal [CAL77] to LAG-3
  • Host species

    Rabbit
  • Specificity

    IHC-P is only recommended for human samples. We obtained negative staining results with mouse.

  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF,IP or WB
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • IHC-P: Human tonsil tissue. Flow Cyt: Human PBMC. Mouse splenocytes treated with ConA.
  • General notes

    This product was previously labelled as Lymphocyte Activation Gene 3.

Properties

Applications

Our Abpromise guarantee covers the use of ab237720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/900. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/500.
  • Application notes
    Is unsuitable for ICC/IF,IP or WB.
  • Target

    • Function

      Involved in lymphocyte activation. Binds to HLA class-II antigens.
    • Tissue specificity

      On cell surface of activated NK and T-lymphocytes.
    • Sequence similarities

      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD223 antibody
      • CD223 antigen antibody
      • FDC protein antibody
      • LAG-3 antibody
      • Lag3 antibody
      • LAG3_HUMAN antibody
      • Lymphocyte activating 3 antibody
      • Lymphocyte activation gene 3 antibody
      • Lymphocyte activation gene 3 protein antibody
      • Protein FDC antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LAG-3 with ab237720 at 1/900 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the human tonsil is observed. The section was incubated with ab237720 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 30 mins.

    • Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 with ab237720 at 0.25 µg/ml in immunohistochemical analysis.

    • Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized human peripheral blood mononuclear cell (PBMC) treated with 10μg/ml PHA for 2 days, labeling LAG-3 with ab237720 at 1/500 (right panel) compared with a Rabbit monoclonal IgG (ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD3 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min followed by intracellular staining with rabbit IgG (Left) and ab237720 (Right).

    • Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse splenocytes treated with 2.5μg/ml Concanavalin A (ConA) for 3 days, labeling LAG-3 with ab237720 at 1/500 (right panel) compared with a Rabbit monoclonal IgG (ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD3 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min followed by intracellular staining with rabbit IgG (Left) and ab237720 (Right).

    References

    ab237720 has not yet been referenced specifically in any publications.

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