Overview

  • Product name

    Anti-LAG-3 antibody [SP346] - BSA and Azide free
    See all LAG-3 primary antibodies
  • Description

    Rabbit monoclonal [SP346] to LAG-3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (C terminal). The exact sequence is proprietary.
    Database link: P18627

  • Positive control

    • IHC-P: Human tonsil, lymphoma, Human Hodgkin’s Lymphoma, and tissue.
  • General notes

    ab241407 is the carrier-free version of ab227706 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab241407 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Lymphocyte Activation Gene 3.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab241407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary antibody incubation for 10 minutes at room temperature.

Target

  • Function

    Involved in lymphocyte activation. Binds to HLA class-II antigens.
  • Tissue specificity

    On cell surface of activated NK and T-lymphocytes.
  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD223 antibody
    • CD223 antigen antibody
    • FDC protein antibody
    • LAG-3 antibody
    • Lag3 antibody
    • LAG3_HUMAN antibody
    • Lymphocyte activating 3 antibody
    • Lymphocyte activation gene 3 antibody
    • Lymphocyte activation gene 3 protein antibody
    • Protein FDC antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling LAG-3 with Purified ab227706 at 1/100 dilution (2.34). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Citrate buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227706)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin�s Lymphoma tissue sections labeling LAG-3 with Purified ab227706 at 1/100 dilution (2.34). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Citrate buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227706)
  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 using ab227706 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227706)

  • Formalin-fixed, paraffin-embedded human lymphoma tissue stained for LAG-3 using ab227706 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227706)

References

ab241407 has not yet been referenced specifically in any publications.

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