Overview

  • Product name

    Anti-LAG-3 antibody [SP346] - C-terminal
    See all LAG-3 primary antibodies
  • Description

    Rabbit monoclonal [SP346] to LAG-3 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human LAG-3 (C terminal).
    Database link: P18627

  • Positive control

    • IHC-P: Human tonsil, lymphoma, Human Hodgkin’s Lymphoma, and tissue.
  • General notes

    This product was previously labelled as Lymphocyte Activation Gene 3.

Properties

Applications

Our Abpromise guarantee covers the use of ab227706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary antibody incubation for 10 minutes at room temperature.

Target

  • Function

    Involved in lymphocyte activation. Binds to HLA class-II antigens.
  • Tissue specificity

    On cell surface of activated NK and T-lymphocytes.
  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD223 antibody
    • CD223 antigen antibody
    • FDC protein antibody
    • LAG-3 antibody
    • Lag3 antibody
    • LAG3_HUMAN antibody
    • Lymphocyte activating 3 antibody
    • Lymphocyte activation gene 3 antibody
    • Lymphocyte activation gene 3 protein antibody
    • Protein FDC antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling LAG-3 with Purified ab227706 at 1/100 dilution (2.34). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Citrate buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin�s Lymphoma tissue sections labeling LAG-3 with Purified ab227706 at 1/100 dilution (2.34). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Citrate buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 using ab227706 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lymphoma tissue stained for LAG-3 using ab227706 at 1/100 dilution in immunohistochemical analysis.

References

ab227706 has not yet been referenced specifically in any publications.

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