Overview

  • Product name

    Anti-LAIR1 antibody [113] - Low endotoxin, Azide free
    See all LAIR1 primary antibodies
  • Description

    Armenian Hamster monoclonal [113] to LAIR1 - Low endotoxin, Azide free
  • Host species

    Armenian hamster
  • Tested applications

    Suitable for: Functional Studies, Flow Cyt, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • Mouse splenocytes.
  • General notes

    Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by the LAL assay.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    113
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab171239 can be used at less than or equal to 0.25μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10e5 to 10e8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
IP Use at an assay dependent concentration.

Target

  • Function

    Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, downregulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.
  • Tissue specificity

    Expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells. Highly expressed in naive T-cells and B-cells but no expression on germinal center B-cells. Abnormally low expression in naive B-cells from HIV-1 infected patients. Very low expression in NK cells from a patient with chronic active Epstein-Barr virus infection.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Developmental stage

    Complete loss of expression when naive B-cells proliferates and differentiates into Ig-producing plasma cells under in vitro stimulation.
  • Domain

    ITIM (immunoreceptor tyrosine-based inhibitor motif) motif is a cytoplasmic motif present in 2 copies in the intracellular part of LAIR1. When phosphorylated, ITIM motif can bind the SH2 domain of several SH2-containing phosphatases, leading to down-regulation of cell activation.
  • Post-translational
    modifications

    Phosphorylation at Tyr-251 and Tyr-281 activates it. May be phosphorylated by LCK.
    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5133400O11Rik antibody
    • CD305 antibody
    • D7Bwg0421e antibody
    • hLAIR1 antibody
    • LAIR 1 antibody
    • LAIR-1 antibody
    • LAIR1 antibody
    • LAIR1 protein antibody
    • LAIR1_HUMAN antibody
    • Leukocyte associated Ig like receptor 1 antibody
    • Leukocyte associated immunoglobulin like receptor 1 antibody
    • Leukocyte-associated immunoglobulin-like receptor 1 antibody
    see all

Images

  • Flow cytometry analysis of C57B1/6 splenocytes (total viable F4/80 positive cells) labelling LAIR1 with ab171239 at 0.125µg (blue line) and with Purified Armenian Hamster IgG control (purple line), followed by anti-Armenian Hamster IgG PE.

References

This product has been referenced in:

  • Kang X  et al. The ITIM-containing receptor LAIR1 is essential for acute myeloid leukaemia development. Nat Cell Biol 17:665-77 (2015). WB . Read more (PubMed: 25915125) »
See 1 Publication for this product

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