Overview

  • Product name

  • Description

    Goat polyclonal to LAIR1
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human LAIR1 aa 69-81 (internal sequence) (Cysteine residue). (NP_002278.1; NP_068352.1).
    Sequence:

    NDTEDVSQASPSE


    Database link: Q6GTX8

  • Positive control

    • Human tonsil tissue; Human lymph node lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 31 kDa).

Target

  • Function

    Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, downregulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.
  • Tissue specificity

    Expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells. Highly expressed in naive T-cells and B-cells but no expression on germinal center B-cells. Abnormally low expression in naive B-cells from HIV-1 infected patients. Very low expression in NK cells from a patient with chronic active Epstein-Barr virus infection.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Developmental stage

    Complete loss of expression when naive B-cells proliferates and differentiates into Ig-producing plasma cells under in vitro stimulation.
  • Domain

    ITIM (immunoreceptor tyrosine-based inhibitor motif) motif is a cytoplasmic motif present in 2 copies in the intracellular part of LAIR1. When phosphorylated, ITIM motif can bind the SH2 domain of several SH2-containing phosphatases, leading to down-regulation of cell activation.
  • Post-translational
    modifications

    Phosphorylation at Tyr-251 and Tyr-281 activates it. May be phosphorylated by LCK.
    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5133400O11Rik antibody
    • CD305 antibody
    • D7Bwg0421e antibody
    • hLAIR1 antibody
    • LAIR 1 antibody
    • LAIR-1 antibody
    • LAIR1 antibody
    • LAIR1 protein antibody
    • LAIR1_HUMAN antibody
    • Leukocyte associated Ig like receptor 1 antibody
    • Leukocyte associated immunoglobulin like receptor 1 antibody
    • Leukocyte-associated immunoglobulin-like receptor 1 antibody
    see all

Images

  • Anti-LAIR1 antibody (ab189412) at 2 µg/ml + Human Lymph Node lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 31 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling LAIR1 with ab189412 at 3.75µg/ml.

References

ab189412 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189412.
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