Key features and details
- Goat polyclonal to LAIR1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LAIR1 antibody
See all LAIR1 primary antibodies
DescriptionGoat polyclonal to LAIR1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- Human tonsil tissue; Human lymph node lysate.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 31 kDa).|
FunctionFunctions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, downregulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.
Tissue specificityExpressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells. Highly expressed in naive T-cells and B-cells but no expression on germinal center B-cells. Abnormally low expression in naive B-cells from HIV-1 infected patients. Very low expression in NK cells from a patient with chronic active Epstein-Barr virus infection.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Developmental stageComplete loss of expression when naive B-cells proliferates and differentiates into Ig-producing plasma cells under in vitro stimulation.
DomainITIM (immunoreceptor tyrosine-based inhibitor motif) motif is a cytoplasmic motif present in 2 copies in the intracellular part of LAIR1. When phosphorylated, ITIM motif can bind the SH2 domain of several SH2-containing phosphatases, leading to down-regulation of cell activation.
modificationsPhosphorylation at Tyr-251 and Tyr-281 activates it. May be phosphorylated by LCK.
Cellular localizationCell membrane.
- Information by UniProt
- 5133400O11Rik antibody
- CD305 antibody
- D7Bwg0421e antibody
Anti-LAIR1 antibody (ab189412) at 2 µg/ml + Human Lymph Node lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 31 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling LAIR1 with ab189412 at 3.75µg/ml.
ab189412 has not yet been referenced specifically in any publications.