Overview

  • Product name

    Anti-LAIR1 antibody [NKTA255] (PerCP/Cy5.5®)
    See all LAIR1 primary antibodies
  • Description

    Mouse monoclonal [NKTA255] to LAIR1 (PerCP/Cy5.5®)
  • Host species

    Mouse
  • Conjugation

    PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human LAIR1. Activated NK cells and CD3- thymocytes.

  • Positive control

    • Flow Cytometry: Human peripheral blood.
  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.0975% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    ab234263 is conjugated with tandem dye PerCP-Cy™5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
  • Clonality

    Monoclonal
  • Clone number

    NKTA255
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab234263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 4µl for 106 cells.

(or 100 μL whole blood)

Target

  • Function

    Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, downregulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.
  • Tissue specificity

    Expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells. Highly expressed in naive T-cells and B-cells but no expression on germinal center B-cells. Abnormally low expression in naive B-cells from HIV-1 infected patients. Very low expression in NK cells from a patient with chronic active Epstein-Barr virus infection.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Developmental stage

    Complete loss of expression when naive B-cells proliferates and differentiates into Ig-producing plasma cells under in vitro stimulation.
  • Domain

    ITIM (immunoreceptor tyrosine-based inhibitor motif) motif is a cytoplasmic motif present in 2 copies in the intracellular part of LAIR1. When phosphorylated, ITIM motif can bind the SH2 domain of several SH2-containing phosphatases, leading to down-regulation of cell activation.
  • Post-translational
    modifications

    Phosphorylation at Tyr-251 and Tyr-281 activates it. May be phosphorylated by LCK.
    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5133400O11Rik antibody
    • CD305 antibody
    • D7Bwg0421e antibody
    • hLAIR1 antibody
    • LAIR 1 antibody
    • LAIR-1 antibody
    • LAIR1 antibody
    • LAIR1 protein antibody
    • LAIR1_HUMAN antibody
    • Leukocyte associated Ig like receptor 1 antibody
    • Leukocyte associated immunoglobulin like receptor 1 antibody
    • Leukocyte-associated immunoglobulin-like receptor 1 antibody
    see all

Images

  • Flow cytometric analysis of surface stained human peripheral blood cells, labeling LAIR1 with ab234263 at 4 μL per 106 cells. Lymphocyte gate.

References

ab234263 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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