Overview

  • Product name

    Anti-LAMA antibody [CL3087]
  • Description

    Mouse monoclonal [CL3087] to LAMA
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human LAMA aa 640-654. The exact sequence is proprietary.
    Sequence:

    LVPENFQDFHSKRQI


    Database link: P25391

  • Epitope

    ab210954 binds to an epitope located within the peptide sequence LVPENFQDFHSKRQI (aa 640 - 654)
  • Positive control

    • Human recombinant LAMA; human testis tissue lysate; human lymph node and testis tissues.
  • General notes

     This product was previously labelled as Laminin alpha 1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab210954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 337 kDa.

Target

  • Relevance

    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix, basement membrane.
  • Database links

  • Alternative names

    • LAMA antibody
    • LAMA1 antibody
    • Laminin A antibody
    • Laminin A chain antibody
    • laminin subunit alpha 1 antibody
    • laminin, alpha 1 antibody
    • laminin-3 subunit alpha antibody
    • PTBHS antibody
    • S-LAM-alpha antibody
    • S-laminin subunit alpha antibody
    see all

Images

  • All lanes : Anti-LAMA antibody [CL3087] (ab210954) at 1/500 dilution

    Lane 1 : Recombinant protein fragment of human Laminin 111
    Lane 2 : Recombinant protein fragment of human Laminin 121
    Lane 3 : Recombinant protein fragment of human Laminin 221
    Lane 4 : Recombinant protein fragment of human Laminin 332
    Lane 5 : Recombinant protein fragment of human Laminin 411
    Lane 6 : Recombinant protein fragment of human Laminin 521

    Developed using the ECL technique.

    Predicted band size: 337 kDa

  • Anti-LAMA antibody [CL3087] (ab210954) at 1/500 dilution + Human testis tissue lysate

    Developed using the ECL technique.

    Predicted band size: 337 kDa

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue, labeling LAMA using ab210954 at a 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human lymph node tissue (negative control), labeling LAMA using ab210954 at a 1/200 dilution.

References

ab210954 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Kidney)
Permeabilization
Yes - 0.1% Triton X-100 in blocking solution, 0.03% Triton X-100 in primary and secondary antibody solutions
Specification
Kidney
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Methanol

Jared Watters

Verified customer

Submitted Sep 07 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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