Anti-LAMA1 antibody [CL3087] (ab210954)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (ab210954)](https://www.abcam.com/ps/products/210/ab210954/Images/ab210954-260028-anti-laminin-alpha-1-antibody-cl3087-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (ab210954)")
Key features and details
- Mouse monoclonal [CL3087] to LAMA1
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-LAMA1 antibody [CL3087]
See all LAMA1 primary antibodies -
Description
Mouse monoclonal [CL3087] to LAMA1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human LAMA aa 600-700. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P25391 -
Epitope
ab210954 binds to an epitope located within the peptide sequence LVPENFQDFHSKRQI (aa 640 - 654) -
Positive control
- WB: HeLa cell lysate; Human testis tissue lysate. IHC-P: Human lymph node and testis tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL3087 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/500 - 1/1000. Predicted molecular weight: 337 kDa.
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| Notes |
|---|
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/500 - 1/1000. Predicted molecular weight: 337 kDa. |
Target
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Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
Involvement in disease
Poretti-Boltshauser syndrome -
Sequence similarities
Contains 17 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 2 laminin IV type A domains.
Contains 1 laminin N-terminal domain. -
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI, IV and G are globular. -
Post-translational
modificationsTyrosine phosphorylated by PKDCC/VLK. -
Cellular localization
Secreted, extracellular space, extracellular matrix, basement membrane. Major component. - Information by UniProt
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Database links
- Entrez Gene: 284217 Human
- Entrez Gene: 16772 Mouse
- Entrez Gene: 316758 Rat
- Omim: 150320 Human
- SwissProt: P25391 Human
- SwissProt: P19137 Mouse
- Unigene: 270364 Human
- Unigene: 303386 Mouse
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Alternative names
- LAMA antibody
- LAMA1 antibody
- LAMA1_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (ab210954)
Immunohistochemical analysis of paraffin-embedded Human testis tissue, labeling LAMA1 using ab210954 at a 1/200 dilution.
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![Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)](https://www.abcam.com/ps/products/210/ab210954/Images/ab210954-397648-anti-lama-antibody-cl3087-western-blot-human-knockout-lysate.jpg)
Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)All lanes : Anti-LAMA1 antibody [CL3087] (ab210954) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : LAMA1 knockout HeLa cell lysate
Lane 3 : Human testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 337 kDa
Observed band size: 337 kDaLanes 1-3: Merged signal (red and green). Green - ab210954 observed at 337 kDa. Red - loading control ab129002 observed at 124 kDa.
ab210954 Anti-LAMA1 antibody [CL3087] was shown to specifically react with LAMA1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264991 (knockout cell lysate ab258937) was used. Wild-type and LAMA1 knockout samples were subjected to SDS-PAGE. ab210954 and Anti-Vinculin antibody [EPR8185] (ab129002) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (ab210954)](https://www.abcam.com/ps/products/210/ab210954/Images/ab210954-260027-anti-laminin-alpha-1-antibody-cl3087-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (ab210954)Immunohistochemical analysis of paraffin-embedded Human lymph node tissue (negative control), labeling LAMA1 using ab210954 at a 1/200 dilution.
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![Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)](https://www.abcam.com/ps/products/210/ab210954/Images/ab210954-260030-anti-laminin-alpha-1-antibody-cl3087-western-blot.jpg)
Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)All lanes : Anti-LAMA1 antibody [CL3087] (ab210954) at 1/500 dilution
Lane 1 : Recombinant protein fragment of human Laminin 111
Lane 2 : Recombinant protein fragment of human Laminin 121
Lane 3 : Recombinant protein fragment of human Laminin 221
Lane 4 : Recombinant protein fragment of human Laminin 332
Lane 5 : Recombinant protein fragment of human Laminin 411
Lane 6 : Recombinant protein fragment of human Laminin 521
Developed using the ECL technique.
Predicted band size: 337 kDa -
![Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)](https://www.abcam.com/ps/products/210/ab210954/Images/ab210954-260029-anti-laminin-alpha-1-antibody-cl3087-western-blot.jpg)
Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)Anti-LAMA1 antibody [CL3087] (ab210954) at 1/500 dilution + Human testis tissue lysate
Developed using the ECL technique.
Predicted band size: 337 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab210954 has not yet been referenced specifically in any publications.