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  1. Link

    lama1-antibody-cl3087-ab210954.pdf

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Signal Transduction Cytoskeleton / ECM Basal Lamina
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Validated using a knockout cell line

Anti-LAMA1 antibody [CL3087] (ab210954)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (ab210954)
  • Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (ab210954)
  • Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)
  • Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)

Key features and details

  • Mouse monoclonal [CL3087] to LAMA1
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

You may also be interested in

Primary
Product image
Anti-Laminin antibody (ab11575)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-LAMA1 antibody [CL3087]
    See all LAMA1 primary antibodies
  • Description

    Mouse monoclonal [CL3087] to LAMA1
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human LAMA aa 600-700. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P25391

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Epitope

    ab210954 binds to an epitope located within the peptide sequence LVPENFQDFHSKRQI (aa 640 - 654)
  • Positive control

    • WB: HeLa cell lysate; Human testis tissue lysate. IHC-P: Human lymph node and testis tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    CL3087
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Basal Lamina
    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Other ECM Proteins
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab210954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/500 - 1/1000. Predicted molecular weight: 337 kDa.
Notes
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/500 - 1/1000. Predicted molecular weight: 337 kDa.

Target

  • Function

    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Involvement in disease

    Poretti-Boltshauser syndrome
  • Sequence similarities

    Contains 17 laminin EGF-like domains.
    Contains 5 laminin G-like domains.
    Contains 2 laminin IV type A domains.
    Contains 1 laminin N-terminal domain.
  • Domain

    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI, IV and G are globular.
  • Post-translational
    modifications

    Tyrosine phosphorylated by PKDCC/VLK.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix, basement membrane. Major component.
  • Target information above from: UniProt accession P25391 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 284217 Human
    • Entrez Gene: 16772 Mouse
    • Entrez Gene: 316758 Rat
    • Omim: 150320 Human
    • SwissProt: P25391 Human
    • SwissProt: P19137 Mouse
    • Unigene: 270364 Human
    • Unigene: 303386 Mouse
    • Alternative names

      • LAMA antibody
      • LAMA1 antibody
      • LAMA1_HUMAN antibody
      • Laminin A antibody
      • Laminin A chain antibody
      • laminin subunit alpha 1 antibody
      • Laminin subunit alpha-1 antibody
      • laminin, alpha 1 antibody
      • Laminin-1 subunit alpha antibody
      • Laminin-3 subunit alpha antibody
      • PTBHS antibody
      • S-LAM alpha antibody
      • S-LAM-alpha antibody
      • S-laminin subunit alpha antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (ab210954)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (ab210954)

      Immunohistochemical analysis of paraffin-embedded Human testis tissue, labeling LAMA1 using ab210954 at a 1/200 dilution.

    • Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)
      Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)
      All lanes : Anti-LAMA1 antibody [CL3087] (ab210954) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : LAMA1 knockout HeLa cell lysate
      Lane 3 : Human testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution

      Predicted band size: 337 kDa
      Observed band size: 337 kDa



      Lanes 1-3: Merged signal (red and green). Green - ab210954 observed at 337 kDa. Red - loading control ab129002 observed at 124 kDa.

      ab210954 Anti-LAMA1 antibody [CL3087] was shown to specifically react with LAMA1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264991 (knockout cell lysate ab258937) was used. Wild-type and LAMA1 knockout samples were subjected to SDS-PAGE. ab210954 and Anti-Vinculin antibody [EPR8185] (ab129002) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (ab210954)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (ab210954)

      Immunohistochemical analysis of paraffin-embedded Human lymph node tissue (negative control), labeling LAMA1 using ab210954 at a 1/200 dilution.

    • Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)
      Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)
      All lanes : Anti-LAMA1 antibody [CL3087] (ab210954) at 1/500 dilution

      Lane 1 : Recombinant protein fragment of human Laminin 111
      Lane 2 : Recombinant protein fragment of human Laminin 121
      Lane 3 : Recombinant protein fragment of human Laminin 221
      Lane 4 : Recombinant protein fragment of human Laminin 332
      Lane 5 : Recombinant protein fragment of human Laminin 411
      Lane 6 : Recombinant protein fragment of human Laminin 521

      Developed using the ECL technique.

      Predicted band size: 337 kDa

    • Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)
      Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)
      Anti-LAMA1 antibody [CL3087] (ab210954) at 1/500 dilution + Human testis tissue lysate

      Developed using the ECL technique.

      Predicted band size: 337 kDa

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab210954? Please let us know so that we can cite the reference in this datasheet.

    ab210954 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Frozen sections) abreview for Anti-Laminin alpha 1 antibody [CL3087]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Kidney)
    Permeabilization
    Yes - 0.1% Triton X-100 in blocking solution, 0.03% Triton X-100 in primary and secondary antibody solutions
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Methanol
    Read More

    Jared Watters

    Verified customer

    Submitted Sep 07 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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