Key features and details
- Mouse monoclonal [CL3112] to LAMA3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-LAMA3 antibody [CL3112]
See all LAMA3 primary antibodies
DescriptionMouse monoclonal [CL3112] to LAMA3
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- IHC-P: Human stomach, colon and oral mucosa tissue. WB: Human Lamin-332 recombinant protein.
This product was previously labelled as Laminin subunit alpha-3, Laminin 5 alpha 3
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein A purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab242197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 367 kDa.|
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes.
Tissue specificitySkin; respiratory, urinary, and digestive epithelia and in other specialized tissues with prominent secretory or protective functions. Epithelial basement membrane, and epithelial cell tongue that migrates into a wound bed. A differential and focal expression of the subunit alpha-3 is observed in the CNS.
Involvement in diseaseDefects in LAMA3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic.
Defects in LAMA3 are the cause of laryngoonychocutaneous syndrome (LOCS) [MIM:245660]. LOCS is an autosomal recessive epithelial disorder confined to the Punjabi Muslim population. The condition is characterized by cutaneous erosions, nail dystrophy and exuberant vascular granulation tissue in certain epithelia, especially conjunctiva and larynx.
Sequence similaritiesContains 15 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain.
DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain G is globular.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. Major component.
- Information by UniProt
- E170 antibody
- Epiligrin 170 kDa subunit antibody
- Epiligrin subunit alpha antibody
Paraffin-embedded human oral mucosa tissue stained for LAMA3 using ab242197 at 1 µg/mL in immunohistochemcial analysis.
Paraffin-embedded human colon tissue stained for LAMA3 using ab242197 at 1 µg/mL in immunohistochemcial analysis.
Paraffin-embedded human stomach tissue stained for LAMA3 using ab242197 at 1 µg/mL in immunohistochemcial analysis.
Paraffin-embedded human lymph node tissue stained for LAMA3 using ab242197 at 1 µg/mL in immunohistochemcial analysis.
All lanes : Anti-LAMA3 antibody [CL3112] (ab242197) at 1 µg/ml
Lane 1 : Purified human Lamin-332 recombinant protein
Lane 2 : Purified human Lamin-421 recombinant protein
Lane 3 : Purified human Lamin-511 recombinant protein
Lane 4 : Purified human Lamin-121 recombinant protein
Lane 5 : Purified human Lamin-221 recombinant protein
Predicted band size: 367 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab242197 has been referenced in 1 publication.
- Xu SF et al. Long Non-coding RNA LINC00628 Interacts Epigenetically with the LAMA3 Promoter and Contributes to Lung Adenocarcinoma. Mol Ther Nucleic Acids 18:166-182 (2019). PubMed: 31557618