Overview

  • Product name

    Anti-LAMA3 antibody [EPR8266]
    See all LAMA3 primary antibodies
  • Description

    Rabbit monoclonal [EPR8266] to LAMA3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human LAMA3.
    Database link: Q16787

  • Positive control

    • HeLa, HepG2, A431, Human skin and SH-SY5Y lysates; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Laminin subunit alpha-3, Laminin 5 alpha 3

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 367 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
      Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes.
    • Tissue specificity

      Skin; respiratory, urinary, and digestive epithelia and in other specialized tissues with prominent secretory or protective functions. Epithelial basement membrane, and epithelial cell tongue that migrates into a wound bed. A differential and focal expression of the subunit alpha-3 is observed in the CNS.
    • Involvement in disease

      Defects in LAMA3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic.
      Defects in LAMA3 are the cause of laryngoonychocutaneous syndrome (LOCS) [MIM:245660]. LOCS is an autosomal recessive epithelial disorder confined to the Punjabi Muslim population. The condition is characterized by cutaneous erosions, nail dystrophy and exuberant vascular granulation tissue in certain epithelia, especially conjunctiva and larynx.
    • Sequence similarities

      Contains 15 laminin EGF-like domains.
      Contains 5 laminin G-like domains.
      Contains 1 laminin IV type A domain.
      Contains 1 laminin N-terminal domain.
    • Domain

      The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
      Domain G is globular.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix > basement membrane. Major component.
    • Information by UniProt
    • Database links

    • Alternative names

      • E170 antibody
      • Epiligrin 170 kDa subunit antibody
      • Epiligrin subunit alpha antibody
      • Kalinin B1 chain antibody
      • Kalinin subunit alpha antibody
      • LAMA3 antibody
      • LAMA3_HUMAN antibody
      • LAMB3 antibody
      • LAMC2 antibody
      • Laminin gamma 2 antibody
      • Laminin 5 beta 3 antibody
      • Laminin 5 gamma 2 subunit antibody
      • Laminin beta 3 antibody
      • Laminin subunit alpha-3 antibody
      • Laminin subunit beta 3 antibody
      • Laminin subunit gamma 2 antibody
      • Laminin-5 subunit alpha antibody
      • Laminin-6 subunit alpha antibody
      • Laminin-7 subunit alpha antibody
      • Nicein subunit alpha antibody
      see all

    Images

    • Immunofluorescent analysis of HepG2 cells labeling LAMA3 with ab151715 at 1/100 dilution.

    • All lanes : Anti-LAMA3 antibody [EPR8266] (ab151715) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : Human skin lysate
      Lane 5 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 367 kDa

    References

    ab151715 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab151715.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up