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    lama3-antibody-epr8266-ab151715.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Extracellular Matrix
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-LAMA3 antibody [EPR8266] (ab151715)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-LAMA3 antibody [EPR8266] (ab151715)
  • Western blot - Anti-LAMA3 antibody [EPR8266] (ab151715)
  • Western blot - Anti-LAMA3 antibody [EPR8266] (ab151715)
  • Anti-LAMA3 antibody [EPR8266] (ab151715)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8266] to LAMA3
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-LAMA3 antibody [EPR8266]
    See all LAMA3 primary antibodies
  • Description

    Rabbit monoclonal [EPR8266] to LAMA3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human LAMA3.
    Database link: Q16787

  • Positive control

    • HeLa, HepG2, A431, Human skin and SH-SY5Y lysates; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Laminin subunit alpha-3, Laminin 5 alpha 3

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8266
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Extracellular Matrix
    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Signal Transduction
    • Cytoskeleton / ECM
    • Basal Lamina
    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other

Associated products

  • Alternative Versions

    • Anti-LAMA3 antibody [EPR8266] - BSA and Azide free (ab249033)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human LAMA3 knockout HeLa cell line (ab265663)
  • KO cell lysates

    • Human LAMA3 knockout HeLa cell lysate (ab257497)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human LAMA3 protein (ab158810)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 367 kDa.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 367 kDa.
ICC/IF
1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
      Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes.
    • Tissue specificity

      Skin; respiratory, urinary, and digestive epithelia and in other specialized tissues with prominent secretory or protective functions. Epithelial basement membrane, and epithelial cell tongue that migrates into a wound bed. A differential and focal expression of the subunit alpha-3 is observed in the CNS.
    • Involvement in disease

      Defects in LAMA3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic.
      Defects in LAMA3 are the cause of laryngoonychocutaneous syndrome (LOCS) [MIM:245660]. LOCS is an autosomal recessive epithelial disorder confined to the Punjabi Muslim population. The condition is characterized by cutaneous erosions, nail dystrophy and exuberant vascular granulation tissue in certain epithelia, especially conjunctiva and larynx.
    • Sequence similarities

      Contains 15 laminin EGF-like domains.
      Contains 5 laminin G-like domains.
      Contains 1 laminin IV type A domain.
      Contains 1 laminin N-terminal domain.
    • Domain

      The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
      Domain G is globular.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix > basement membrane. Major component.
    • Target information above from: UniProt accession Q16787 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3909 Human
      • Entrez Gene: 3914 Human
      • Entrez Gene: 3918 Human
      • Omim: 150310 Human
      • Omim: 226650 Human
      • Omim: 226700 Human
      • SwissProt: Q13751 Human
      • SwissProt: Q13753 Human
      • SwissProt: Q16787 Human
      • Unigene: 436367 Human
      see all
    • Alternative names

      • E170 antibody
      • Epiligrin 170 kDa subunit antibody
      • Epiligrin subunit alpha antibody
      • Kalinin B1 chain antibody
      • Kalinin subunit alpha antibody
      • LAMA3 antibody
      • LAMA3_HUMAN antibody
      • LAMB3 antibody
      • LAMC2 antibody
      • Laminin gamma 2 antibody
      • Laminin 5 beta 3 antibody
      • Laminin 5 gamma 2 subunit antibody
      • Laminin beta 3 antibody
      • Laminin subunit alpha-3 antibody
      • Laminin subunit beta 3 antibody
      • Laminin subunit gamma 2 antibody
      • Laminin-5 subunit alpha antibody
      • Laminin-6 subunit alpha antibody
      • Laminin-7 subunit alpha antibody
      • Nicein subunit alpha antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-LAMA3 antibody [EPR8266] (ab151715)
      Immunocytochemistry/ Immunofluorescence - Anti-LAMA3 antibody [EPR8266] (ab151715)

      Immunofluorescent analysis of HepG2 cells labeling LAMA3 with ab151715 at 1/100 dilution.

    • Western blot - Anti-LAMA3 antibody [EPR8266] (ab151715)
      Western blot - Anti-LAMA3 antibody [EPR8266] (ab151715)
      All lanes : Anti-LAMA3 antibody [EPR8266] (ab151715) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : LAMA3 knockout HeLa cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 367 kDa
      Observed band size: 367 kDa



      Lanes 1 - 3: Merged signal (red and green). Green - ab151715 observed at 367 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

      ab151715 was shown to react with LAMA3 in wild-type HeLa cells in western blot with loss of signal observed in LAMA3 knockout cell line ab265663 (LAMA3 knockout cell lysate ab257497). Wild-type and LAMA3 knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab151715 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-LAMA3 antibody [EPR8266] (ab151715)
      Western blot - Anti-LAMA3 antibody [EPR8266] (ab151715)
      All lanes : Anti-LAMA3 antibody [EPR8266] (ab151715) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : Human skin lysate
      Lane 5 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 367 kDa

    • Anti-LAMA3 antibody [EPR8266] (ab151715)
      Anti-LAMA3 antibody [EPR8266] (ab151715)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab151715? Please let us know so that we can cite the reference in this datasheet.

    ab151715 has been referenced in 1 publication.

    • Efraim Y  et al. Alterations in corneal biomechanics underlie early stages of autoimmune-mediated dry eye disease. J Autoimmun 114:102500 (2020). PubMed: 32565048

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