Recombinant Anti-LAMA3 antibody [EPR8266] (ab151715)
![Immunocytochemistry/ Immunofluorescence - Anti-LAMA3 antibody [EPR8266] (ab151715)](https://www.abcam.com/ps/products/151/ab151715/Images/ab151715-158763-anti-laminin-5-alpha-3-antibody-epr8266-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-LAMA3 antibody [EPR8266] (ab151715)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8266] to LAMA3
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-LAMA3 antibody [EPR8266]
See all LAMA3 primary antibodies -
Description
Rabbit monoclonal [EPR8266] to LAMA3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human LAMA3.
Database link: Q16787 -
Positive control
- HeLa, HepG2, A431, Human skin and SH-SY5Y lysates; HepG2 cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Laminin subunit alpha-3, Laminin 5 alpha 3
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8266 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 367 kDa.
|
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| ICC/IF |
1/100 - 1/250.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 367 kDa. |
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ICC/IF
1/100 - 1/250. |
Target
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Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes. -
Tissue specificity
Skin; respiratory, urinary, and digestive epithelia and in other specialized tissues with prominent secretory or protective functions. Epithelial basement membrane, and epithelial cell tongue that migrates into a wound bed. A differential and focal expression of the subunit alpha-3 is observed in the CNS. -
Involvement in disease
Defects in LAMA3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic.
Defects in LAMA3 are the cause of laryngoonychocutaneous syndrome (LOCS) [MIM:245660]. LOCS is an autosomal recessive epithelial disorder confined to the Punjabi Muslim population. The condition is characterized by cutaneous erosions, nail dystrophy and exuberant vascular granulation tissue in certain epithelia, especially conjunctiva and larynx. -
Sequence similarities
Contains 15 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain. -
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain G is globular. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. Major component. - Information by UniProt
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Database links
- Entrez Gene: 3909 Human
- Entrez Gene: 3914 Human
- Entrez Gene: 3918 Human
- Omim: 150310 Human
- Omim: 226650 Human
- Omim: 226700 Human
- SwissProt: Q13751 Human
- SwissProt: Q13753 Human
see all -
Alternative names
- E170 antibody
- Epiligrin 170 kDa subunit antibody
- Epiligrin subunit alpha antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-LAMA3 antibody [EPR8266] (ab151715)
Immunofluorescent analysis of HepG2 cells labeling LAMA3 with ab151715 at 1/100 dilution.
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![Western blot - Anti-LAMA3 antibody [EPR8266] (ab151715)](https://www.abcam.com/ps/products/151/ab151715/Images/ab151715-448262-anti-lama3-antibody-epr8266-western-blot-hela-hela-lama3-knockout-a431.jpg)
Western blot - Anti-LAMA3 antibody [EPR8266] (ab151715)All lanes : Anti-LAMA3 antibody [EPR8266] (ab151715) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : LAMA3 knockout HeLa cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 367 kDa
Observed band size: 367 kDaLanes 1 - 3: Merged signal (red and green). Green - ab151715 observed at 367 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab151715 was shown to react with LAMA3 in wild-type HeLa cells in western blot with loss of signal observed in LAMA3 knockout cell line ab265663 (LAMA3 knockout cell lysate ab257497). Wild-type and LAMA3 knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab151715 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-LAMA3 antibody [EPR8266] (ab151715)](https://www.abcam.com/ps/products/151/ab151715/Images/ab151715-158764-anti-laminin-5-alpha-3-antibody-epr8266-western-blot.jpg)
Western blot - Anti-LAMA3 antibody [EPR8266] (ab151715)All lanes : Anti-LAMA3 antibody [EPR8266] (ab151715) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Human skin lysate
Lane 5 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 367 kDa -
![Anti-LAMA3 antibody [EPR8266] (ab151715)](https://www.abcam.com/ps/products/151/ab151715/Images/ab151715-3-benefits-of-recombinant-antibodies.png)
Anti-LAMA3 antibody [EPR8266] (ab151715)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151715 has been referenced in 1 publication.
- Efraim Y et al. Alterations in corneal biomechanics underlie early stages of autoimmune-mediated dry eye disease. J Autoimmun 114:102500 (2020). PubMed: 32565048