Key features and details
- Rabbit polyclonal to LAMB3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-LAMB3 antibody
See all LAMB3 primary antibodies
DescriptionRabbit polyclonal to LAMB3
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human LAMB3 aa 644-930.
- H1299, HeLa, or HepG2 and cell lines. NIH-3T3 and A431 whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 130 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
|ICC/IF||1/100 - 1/1000.|
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificityFound in the basement membranes (major component).
Involvement in diseaseDefects in LAMB3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic.
Defects in LAMB3 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
Sequence similaritiesContains 6 laminin EGF-like domains.
Contains 1 laminin N-terminal domain.
DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain VI is globular.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
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Anti-LAMB3 antibody (ab97765) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 130 kDa
7.5% SDS PAGE
Anti-LAMB3 antibody (ab97765) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 130 kDa
7.5% SDS PAGE
Immunofluorescence analysis of methanol-fixed HeLa, using ab97765 at 1:200.
Immunohistochemical analysis of paraffin-embedded human lung cancer, using ab97765 to detect LAMB3 at 1:250.
ab97765 has not yet been referenced specifically in any publications.