Overview

  • Product name

    Anti-LAMB3 antibody - C-terminal
    See all LAMB3 primary antibodies
  • Description

    Rabbit polyclonal to LAMB3 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human LAMB3 (C terminal). The exact sequence is proprietary.
    Database link: Q13751

  • Positive control

    • WB: A431 whole cell extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 130 kDa.

Target

  • Function

    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificity

    Found in the basement membranes (major component).
  • Involvement in disease

    Defects in LAMB3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic.
    Defects in LAMB3 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • Sequence similarities

    Contains 6 laminin EGF-like domains.
    Contains 1 laminin N-terminal domain.
  • Domain

    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domain VI is globular.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BM600 125KDA antibody
    • Epiligrin subunit bata antibody
    • FLJ99565 antibody
    • Kalinin B1 chain antibody
    • Kalinin subunit beta antibody
    • LAM5 antibody
    • LAMB3 antibody
    • LAMB3_HUMAN antibody
    • Laminin B1k chain antibody
    • Laminin subunit beta-3 antibody
    • Laminin-5 subunit beta antibody
    • LAMNB1 antibody
    • Nicein subunit beta antibody
    see all

Images

  • Anti-LAMB3 antibody - C-terminal (ab228755) at 1/500 dilution + A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 130 kDa



    5% SDS-PAGE gel.

References

ab228755 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228755.
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