Overview

  • Product name

    Anti-LAMB3 antibody [EPR7524]
    See all LAMB3 primary antibodies
  • Description

    Rabbit monoclonal [EPR7524] to LAMB3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human LAMB3 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HACAT and A431 cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 3.92 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.30
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7524
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 130 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
    • Tissue specificity

      Found in the basement membranes (major component).
    • Involvement in disease

      Defects in LAMB3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic.
      Defects in LAMB3 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
    • Sequence similarities

      Contains 6 laminin EGF-like domains.
      Contains 1 laminin N-terminal domain.
    • Domain

      The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
      Domain VI is globular.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • BM600 125KDA antibody
      • Epiligrin subunit bata antibody
      • FLJ99565 antibody
      • Kalinin B1 chain antibody
      • Kalinin subunit beta antibody
      • LAM5 antibody
      • LAMB3 antibody
      • LAMB3_HUMAN antibody
      • Laminin B1k chain antibody
      • Laminin subunit beta-3 antibody
      • Laminin-5 subunit beta antibody
      • LAMNB1 antibody
      • Nicein subunit beta antibody
      see all

    Images

    • All lanes : Anti-LAMB3 antibody [EPR7524] (ab128864) at 1/1000 dilution

      Lane 1 : HACAT cell lysate
      Lane 2 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 130 kDa
      Observed band size: 140 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Monteleon CL  et al. Establishing epithelial glandular polarity: interlinked roles for ARF6, Rac1, and the matrix microenvironment. Mol Biol Cell 23:4495-505 (2012). Dog . Read more (PubMed: 23051733) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab128864.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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