Overview

  • Product name

    Anti-LAMB3 antibody [EPR7525]
    See all LAMB3 primary antibodies
  • Description

    Rabbit monoclonal [EPR7525] to LAMB3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human LAMB3 aa 1150 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • HACAT, Raw264.7, PC-12 and A431 cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7525
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 130 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
    • Tissue specificity

      Found in the basement membranes (major component).
    • Involvement in disease

      Defects in LAMB3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic.
      Defects in LAMB3 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
    • Sequence similarities

      Contains 6 laminin EGF-like domains.
      Contains 1 laminin N-terminal domain.
    • Domain

      The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
      Domain VI is globular.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • BM600 125KDA antibody
      • Epiligrin subunit bata antibody
      • FLJ99565 antibody
      • Kalinin B1 chain antibody
      • Kalinin subunit beta antibody
      • LAM5 antibody
      • LAMB3 antibody
      • LAMB3_HUMAN antibody
      • Laminin B1k chain antibody
      • Laminin subunit beta-3 antibody
      • Laminin-5 subunit beta antibody
      • LAMNB1 antibody
      • Nicein subunit beta antibody
      see all

    Images

    • All lanes : Anti-LAMB3 antibody [EPR7525] (ab150385) at 1/1000 dilution

      Lane 1 : HACAT cell lysate
      Lane 2 : Raw264.7 cell lysate
      Lane 3 : PC-12 cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled Gaot anti-Rabbit at 1/2000 dilution

      Predicted band size: 130 kDa

    References

    This product has been referenced in:

    • Young CD  et al. Activating PIK3CA Mutations Induce an Epidermal Growth Factor Receptor (EGFR)/Extracellular Signal-regulated Kinase (ERK) Paracrine Signaling Axis in Basal-like Breast Cancer. Mol Cell Proteomics 14:1959-76 (2015). Read more (PubMed: 25953087) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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