• Product name
    Anti-Lambda Light chain antibody (HRP)
    See all Lambda Light chain primary antibodies
  • Description
    Sheep polyclonal to Lambda Light chain (HRP)
  • Host species
  • Conjugation
  • Specificity
    The unconjugated immunoglobulin gives a single arc when tested by IEP against human serum. Identity has been confirmed by double diffusion (Ouchterlony) against human serum and an anti- HumanLambda of known specificity.
  • Tested applications
    Suitable for: IHC-Fr, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The immunogen used was human lambda-chain, purified from disease state urines and shown to be homogeneous by SDS-PAGE.

  • General notes
    The following points should be observed when using this product: 1) Dilutions of the conjugate must be made in a suitable buffer (e.g. 0.15M Phosphate buffered saline,pH7.2) 2) It is recommended that dilutions of the conjugate are made in polypropylene or polyethylene containers. Other materials (e.g. polystyrene, some grades of glass) may be detrimental to the conjugate. 3) Working dilutions of the conjugate should be prepared immediately prior to use. 4) Peroxidase conjugates are adversely affected by the presence of sodium azide, the use of which shouldtherefore be avoided. 5) The addition of Tween 20 (0.05 – 0.1%) to the conjugate diluent buffer is recommended to prevent nonspecificbinding in EIA. It may also be advantageous in other assay systems.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Proclin
    Constituents: 1% BSA, PBS, 10% Stabilzyme
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
  • Isotype
  • Light chain type
  • Research areas


Our Abpromise guarantee covers the use of ab9007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/25 - 1/50.
ELISA Use at an assay dependent dilution. To give an A450nm = 1.0 using wells coated with human serum at 1/2000 dilution and TMB as a substrate: 1/6400 – 1/12800
WB Use at an assay dependent dilution. See Abreview


  • Relevance
    All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
  • Cellular localization
  • Database links
  • Alternative names
    • Bence Jones protein antibody
    • BJP antibody
    • Constant region of lambda light chains antibody
    • Ig lambda chain C regions antibody
    • ig lambda-6 chain C region antibody
    • IGLC 1 antibody
    • IGLC 2 antibody
    • IGLC 3 antibody
    • IGLC antibody
    • IGLC1 antibody
    • IGLC2 antibody
    • IGLC3 antibody
    • IGLC6 antibody
    • IGLV antibody
    • Immunoglobulin lambda constant 1 antibody
    • Immunoglobulin lambda constant regin 1 antibody
    • immunoglobulin lambda gene cluster antibody
    • Immunoglobulin lambda locus antibody
    • Immunoglobulin lambda variable cluster antibody
    • Immunoglobulin: lambda light chain antibody
    • Mcg marker antibody
    • Paraprotein antibody
    see all


  • All lanes : Anti-Lambda Light chain antibody (HRP) (ab9007) at 1/500 dilution

    Lane 1 : Reduced samples (Conditioned medium from HEK293 expressing recombinant Lambda light chain).
    Lane 2 : Non-reduced samples (Conditioned medium from HEK293 expressing recombinant Lambda light chain).

    Observed band size: 170,25 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 30 kDa (possible non-specific binding)

    The 25 kDa band correspond to the reduced form of Lambda light chain. Please refer to abreview for further details.

    See Abreview


ab9007 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Human Recombinant protein (Human antibody expressed in HEK293 cells)
Human antibody expressed in HEK293 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Feb 24 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Recombinant protein (Conditioned medium from HEK293 expressing rec)
Loading amount
0.05 µg
Conditioned medium from HEK293 expressing rec
Gel Running Conditions
Reduced Denaturing (9%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jan 27 2009


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