Anti-Lambda Light chain antibody [LcN-2] (ab187370)
Key features and details
- Mouse monoclonal [LcN-2] to Lambda Light chain
- Suitable for: IHC-P, IP, IHC-Fr, ICC/IF, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Lambda Light chain antibody [LcN-2]
See all Lambda Light chain primary antibodies -
Description
Mouse monoclonal [LcN-2] to Lambda Light chain -
Host species
Mouse -
Specificity
ab187370 is specific to lambda light chain of immunoglobulin and shows no cross-reaction with kappa light chain or any of the five heavy chains. -
Tested applications
Suitable for: IHC-P, IP, IHC-Fr, ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human Lambda Light chain. (Purified Human IgG).
Database link: P01701 -
Positive control
- 293T, Raji or hPBL cell lysates; Human intestine lysate; Human tonsil or spleen tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab187370 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
LcN-2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab187370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IP | Use at 1-2 µg/mg of lysate. | |
| IHC-Fr | Use a concentration of 0.5 - 1 µg/ml. | |
| ICC/IF | Use a concentration of 1 - 2 µg/ml. | |
| Flow Cyt | Use 0.5-1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 23 kDa. |
Target
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Relevance
All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3535 Human
- Entrez Gene: 3546 Human
- Entrez Gene: 8217 Human
- Omim: 147220 Human
- SwissProt: P01701 Human
- SwissProt: P01842 Human
- Unigene: 640434 Human
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Alternative names
- Bence Jones protein antibody
- BJP antibody
- Constant region of lambda light chains antibody
see all
Images
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![Western blot - Anti-Lambda Light chain antibody [LcN-2] (ab187370)](https://www.abcam.com/ps/products/187/ab187370/Images/ab187370-219588-anti-lambda-light-chain-antibody-lcn-2-western-blot.jpg)
Western blot - Anti-Lambda Light chain antibody [LcN-2] (ab187370)Anti-Lambda Light chain antibody [LcN-2] (ab187370) at 1 µg/ml + Human intestine lysate
Predicted band size: 23 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [LcN-2] (ab187370)](https://www.abcam.com/ps/products/187/ab187370/Images/ab187370-219587-anti-lambda-light-chain-antibody-lcn-2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [LcN-2] (ab187370)Immunohistochemical analysis of formalin-fixed, paraffin embedded Human tonsil tissue labeling Lambda Light chain with ab187370 at 1 µg/ml.
Protocols
Datasheets and documents
References (0)
ab187370 has not yet been referenced specifically in any publications.
