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  1. Link

    lambda-light-chain-antibody-lcn-2-ab187370.pdf

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Immunology Immunoglobulins Light Chain Lambda
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Anti-Lambda Light chain antibody [LcN-2] (ab187370)

  • Datasheet
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  • Western blot - Anti-Lambda Light chain antibody [LcN-2] (ab187370)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [LcN-2] (ab187370)

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-Lambda Light chain antibody [LcN-2]
    See all Lambda Light chain primary antibodies
  • Description

    Mouse monoclonal [LcN-2] to Lambda Light chain
  • Host species

    Mouse
  • Specificity

    ab187370 is specific to lambda light chain of immunoglobulin and shows no cross-reaction with kappa light chain or any of the five heavy chains.
  • Tested applications

    Suitable for: IHC-P, IP, IHC-Fr, ICC/IF, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Lambda Light chain. (Purified Human IgG).
    Database link: P01701

  • Positive control

    • 293T, Raji or hPBL cell lysates; Human intestine lysate; Human tonsil or spleen tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab187370 was purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    LcN-2
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Immunoglobulins
    • Light Chain
    • Lambda

Associated products

  • Alternative Versions

    • Anti-Lambda Light chain antibody [LcN-2] - BSA and Azide free (ab233891)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • 293T whole cell lysate (ab95494)
  • Recombinant Protein

    • Recombinant Human Lambda Light chain protein (ab158750)
  • Related Products

    • Recombinant Human Lambda Light chain protein (ab158750)

Applications

Our Abpromise guarantee covers the use of ab187370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 1-2 µg/mg of lysate.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
ICC/IF Use a concentration of 1 - 2 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 23 kDa.

Target

  • Relevance

    All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
  • Cellular localization

    Cytoplasmic
  • Database links

    • Entrez Gene: 3535 Human
    • Entrez Gene: 3546 Human
    • Entrez Gene: 8217 Human
    • Omim: 147220 Human
    • SwissProt: P01701 Human
    • SwissProt: P01842 Human
    • Unigene: 640434 Human
    • Alternative names

      • Bence Jones protein antibody
      • BJP antibody
      • Constant region of lambda light chains antibody
      • Ig lambda chain C regions antibody
      • ig lambda-6 chain C region antibody
      • IGLC 1 antibody
      • IGLC 2 antibody
      • IGLC 3 antibody
      • IGLC antibody
      • IGLC1 antibody
      • IGLC2 antibody
      • IGLC3 antibody
      • IGLC6 antibody
      • IGLV antibody
      • Immunoglobulin lambda constant 1 antibody
      • Immunoglobulin lambda constant regin 1 antibody
      • immunoglobulin lambda gene cluster antibody
      • Immunoglobulin lambda locus antibody
      • Immunoglobulin lambda variable cluster antibody
      • Immunoglobulin: lambda light chain antibody
      • Mcg marker antibody
      • Paraprotein antibody
      see all

    Images

    • Western blot - Anti-Lambda Light chain antibody [LcN-2] (ab187370)
      Western blot - Anti-Lambda Light chain antibody [LcN-2] (ab187370)
      Anti-Lambda Light chain antibody [LcN-2] (ab187370) at 1 µg/ml + Human intestine lysate

      Predicted band size: 23 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [LcN-2] (ab187370)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [LcN-2] (ab187370)

      Immunohistochemical analysis of formalin-fixed, paraffin embedded Human tonsil tissue labeling Lambda Light chain with ab187370 at 1 µg/ml.

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Datasheets and documents

    • Datasheet
  • References

    ab187370 has not yet been referenced specifically in any publications.

    Publishing research using ab187370? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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