Anti-Lambda Light chain antibody [LcN-2] (ab187370)
![Immunocytochemistry - Anti-Lambda Light chain antibody [LcN-2] (ab187370)](https://www.abcam.com/ps/products/187/ab187370/Images/ab187370-3-anti-lambda-light-chain-antibody-lcn-2-immunocytochemistry-ramos-human.jpg "Immunocytochemistry - Anti-Lambda Light chain antibody [LcN-2] (ab187370)")
Key features and details
- Mouse monoclonal [LcN-2] to Lambda Light chain
- Suitable for: IHC-P, ICC, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Lambda Light chain antibody [LcN-2]
See all Lambda Light chain primary antibodies -
Description
Mouse monoclonal [LcN-2] to Lambda Light chain -
Host species
Mouse -
Specificity
ab187370 is specific to lambda light chain of immunoglobulin and shows no cross-reaction with kappa light chain or any of the five heavy chains. -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human Lambda Light chain. (Purified Human IgG).
Database link: P01701 -
Positive control
- WB: Human intestine lysate; IHC-P: Human tonsil tissue; ICC: Ramos cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab187370 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
LcN-2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 0.25 - 0.5 µg/ml.
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
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| ICC |
Use a concentration of 1 - 2 µg/ml.
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| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 23 kDa.
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| Notes |
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IHC-P
Use a concentration of 0.25 - 0.5 µg/ml. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
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ICC
Use a concentration of 1 - 2 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 23 kDa. |
Target
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Relevance
All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3535 Human
- Entrez Gene: 3546 Human
- Entrez Gene: 8217 Human
- Omim: 147220 Human
- SwissProt: P01701 Human
- SwissProt: P01842 Human
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Alternative names
- Bence Jones protein antibody
- BJP antibody
- Constant region of lambda light chains antibody
see all
Images
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Immunocytochemistry - Anti-Lambda Light chain antibody [LcN-2] (ab187370)Immunocytochemistry analysis of Ramos (human Burkitt's lymphoma cell line) cells using ab187370 (AF488-labeled) (Green). DAPI was used to stain the cell nuclei (blue). Imaged using confocal microscopy.
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![Western blot - Anti-Lambda Light chain antibody [LcN-2] (ab187370)](https://www.abcam.com/ps/products/187/ab187370/Images/ab187370-219588-anti-lambda-light-chain-antibody-lcn-2-western-blot.jpg)
Western blot - Anti-Lambda Light chain antibody [LcN-2] (ab187370)Anti-Lambda Light chain antibody [LcN-2] (ab187370) at 1 µg/ml + Human intestine lysate
Predicted band size: 23 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [LcN-2] (ab187370)](https://www.abcam.com/ps/products/187/ab187370/Images/ab187370-219587-anti-lambda-light-chain-antibody-lcn-2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [LcN-2] (ab187370)Immunohistochemical analysis of formalin-fixed, paraffin embedded Human tonsil tissue labeling Lambda Light chain with ab187370 at 1 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab187370 has not yet been referenced specifically in any publications.
