Anti-Lambda Light chain antibody [LcN-2] - BSA and Azide free (ab233891)
![Western blot - Anti-Lambda Light chain antibody [LcN-2] - BSA and Azide free (ab233891)](https://www.abcam.com/ps/products/233/ab233891/Images/ab233891-308033-anti-lambda-light-chain-antibody-lcn-2-western-blot.jpg "Western blot - Anti-Lambda Light chain antibody [LcN-2] - BSA and Azide free (ab233891)")
Key features and details
- Mouse monoclonal [LcN-2] to Lambda Light chain - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Lambda Light chain antibody [LcN-2] - BSA and Azide free
See all Lambda Light chain primary antibodies -
Description
Mouse monoclonal [LcN-2] to Lambda Light chain - BSA and Azide free -
Host species
Mouse -
Specificity
ab233891 is specific to lambda light chain of immunoglobulin and shows no cross-reaction with kappa light chain or any of the five heavy chains. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Lambda Light chain. (Purified Human IgG).
Database link: P01701 -
Positive control
- WB: Human intestinal lysate. IHC-P: Human tonsil tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
LcN-2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 23 kDa.
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| IHC-P |
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate with primary antibody for 30 minutes at room temperature. |
| Notes |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 23 kDa. |
|
IHC-P
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 30 minutes at room temperature. |
Target
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Relevance
All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3535 Human
- Entrez Gene: 3546 Human
- Entrez Gene: 8217 Human
- Omim: 147220 Human
- SwissProt: P01701 Human
- SwissProt: P01842 Human
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Alternative names
- Bence Jones protein antibody
- BJP antibody
- Constant region of lambda light chains antibody
see all
Images
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Western blot - Anti-Lambda Light chain antibody [LcN-2] - BSA and Azide free (ab233891)Anti-Lambda Light chain antibody [LcN-2] - BSA and Azide free (ab233891) at 1 µg/ml + Human intestinal lysate
Predicted band size: 23 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [LcN-2] - BSA and Azide free (ab233891)](https://www.abcam.com/ps/products/233/ab233891/Images/ab233891-308035-anti-lambda-light-chain-antibody-lcn-2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [LcN-2] - BSA and Azide free (ab233891)Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lambda light chain using ab233891 at 0.5 μg/ml in immunohistochemical analysis
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233891 has not yet been referenced specifically in any publications.
