Overview

  • Product name

    Anti-Lambda Light chain antibody, prediluted
    See all Lambda Light chain primary antibodies
  • Description

    Rabbit polyclonal to Lambda Light chain, prediluted
  • Host species

    Rabbit
  • Specificity

    We have data to indicate that this antibody may not cross react with Dog. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Baboon
  • Immunogen

    Lambda light chains purified from human Bence Jones proteins.

  • Positive control

    • Tonsil

Properties

  • Form

    Prediluted
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.60
    Preservative: 0.097% Sodium azide
    Constituent: 7.9% Tris HCl

    Stabilizing agent.
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: Prediluted ready for use. Perform heat mediated antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 10mM citrate buffer pH 6.0 for 10-20 mins, followed by cooling at RT for 20 mins.
  • Target

    • Relevance

      All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
    • Cellular localization

      Cytoplasmic
    • Database links

    • Alternative names

      • Bence Jones protein antibody
      • BJP antibody
      • Constant region of lambda light chains antibody
      • Ig lambda chain C regions antibody
      • ig lambda-6 chain C region antibody
      • IGLC 1 antibody
      • IGLC 2 antibody
      • IGLC 3 antibody
      • IGLC antibody
      • IGLC1 antibody
      • IGLC2 antibody
      • IGLC3 antibody
      • IGLC6 antibody
      • IGLV antibody
      • Immunoglobulin lambda constant 1 antibody
      • Immunoglobulin lambda constant regin 1 antibody
      • immunoglobulin lambda gene cluster antibody
      • Immunoglobulin lambda locus antibody
      • Immunoglobulin lambda variable cluster antibody
      • Immunoglobulin: lambda light chain antibody
      • Mcg marker antibody
      • Paraprotein antibody
      see all

    Images

    • ab74316 staining Lambda Light chain in Rat lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 25°C; antigen retrieval was by heat mediation in EDTA pH9. Samples were incubated with primary antibody for 16 hours at 25°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Cytoplasmic staining of B cells in formalin-fixed, paraffin-embedded human tonsil using ab74316 with a peroxidase-conjugated secondary antibody and a DAB chromogen.

    References

    ab74316 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA, pH9
    Sample
    Rat Tissue sections (Lymph node)
    Specification
    Lymph node
    Permeabilization
    No
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Jul 03 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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