Recombinant
RabMAb

Recombinant Anti-Lambda Light chain antibody [SP147] - BSA and Azide free (ab245750)

Overview

  • Product name

    Anti-Lambda Light chain antibody [SP147] - BSA and Azide free
    See all Lambda Light chain primary antibodies
  • Description

    Rabbit monoclonal [SP147] to Lambda Light chain - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Other Immunogen Type corresponding to Human Lambda Light chain. Free lambda light chain from pooled human IgG lambda myeloma sera.

  • Positive control

    • WB: Human spleen lysate.IHC-P: Human tonsil, thyroid, B-cell lymphoma and follicule lymphoma tissues.
  • General notes

    Ab245750 is the carrier-free version of ab227653. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab245750 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP147
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
IHC-P Use at an assay dependent concentration.

For antigen retrieval: Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

 

Target

  • Relevance

    All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • Bence Jones protein antibody
    • BJP antibody
    • Constant region of lambda light chains antibody
    • Ig lambda chain C regions antibody
    • ig lambda-6 chain C region antibody
    • IGLC 1 antibody
    • IGLC 2 antibody
    • IGLC 3 antibody
    • IGLC antibody
    • IGLC1 antibody
    • IGLC2 antibody
    • IGLC3 antibody
    • IGLC6 antibody
    • IGLV antibody
    • Immunoglobulin lambda constant 1 antibody
    • Immunoglobulin lambda constant regin 1 antibody
    • immunoglobulin lambda gene cluster antibody
    • Immunoglobulin lambda locus antibody
    • Immunoglobulin lambda variable cluster antibody
    • Immunoglobulin: lambda light chain antibody
    • Mcg marker antibody
    • Paraprotein antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human thyroid tissue stained for Lambda Light chain using ab227653 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227653).

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lambda Light chain using ab227653 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227653).

  • Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for Lambda Light chain using ab227653 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227653).

  • Formalin-fixed, paraffin-embedded human follicule lymphoma tissue stained for Lambda Light chain using ab227653 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227653).

References

ab245750 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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