Immunocytochemistry/ Immunofluorescence abreview for Anti-Lamin A + C antibody [EPR4068] (Alexa Fluor® 647)

Inconclusive
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Monocytes)
Permeabilization
Yes - Tween-20
Specification
Monocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/1000

Secondary antibody

Secondary antibody
None used

Additional data

Additional Notes
Mouse macrophages were cultured on a glass coverslip. Cells were permeabiliized with 0.01% Tween-20, and stained for Lamin A/C (green, ab223943), actin(red, phalloidin), and DAPI (blue). Cells could not be fixed with methanol, as it disrupts actin.
Abcam response
Thank you for your honest feedback. We are sorry to hear that the antibody did not work in mouse samples.

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Submitted Oct 29 2018

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