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I'd like to send a feedback for anti-LaminA+C ab5090 [JoL5] I purchased recently from Abcam. I used this antibody to detect Lamin A and Lamin C proteins in protein extracts from heart tissue by immunoblot. My results showed a single band with molecular weight (MW) approximately 53-55kD. According to information in product data sheet this antibody is expected to detect two bands with MW approximately 70kD (Lamin A) and 60kD (Lamin C). I used as a secondary antibody anti-mouse HRP conjugate from BioRad. Ab5090 was used in dilution 1:50. On the WB I have a protein extract from Hela cells as a positive control sample. The antibody detects also a single band in this sample. I was surprised that this antibody does not detect the expected two bands. I'd like to receive a replacement of the antibody if it is possible.
Asked on Nov 09 2004
I have received the following information which I hope will help: JoL5 recognises a growth specific epitope. It is expressed in non dividing cells but is undetectable in growing cells. HeLa is a growing cell. You will need to use ab4789 which detects lamin A/C in all cells. Please let me know if you need further information,
Answered on Nov 17 2004