Overview

  • Product name
    Anti-Lamin B Receptor antibody
    See all Lamin B Receptor primary antibodies
  • Description
    Mouse polyclonal to Lamin B Receptor
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Full length protein, corresponding to amino acids 1-615 of Human Lamin B Receptor (NP_002287.2).

  • Positive control
    • Lamin B Receptor transfected 293T cell lysate; Jurkat cell lysate; Human small intestine tissue; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab169306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa.
IHC-P Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Anchors the lamina and the heterochromatin to the inner nuclear membrane.
  • Involvement in disease
    Defects in LBR are a cause of Pelger-Huet anomaly (PHA) [MIM:169400]. PHA is an autosomal dominant inherited abnormality of neutrophils, characterized by reduced nuclear segmentation and an apparently looser chromatin structure. Heterozygotes show hypolobulated neutrophil nuclei with coarse chromatin. Presumed homozygous individuals have ovoid neutrophil nuclei, as well as varying degrees of developmental delay, epilepsy, and skeletal abnormalities.
    Defects in LBR are the cause of hydrops-ectopic calcification-moth-eaten skeletal dysplasia (HEM) [MIM:215140]; also known as Greenberg skeletal dysplasia. HEM is a rare autosomal recessive chondrodystrophy characterized by early in utero lethality and, therefore, considered to be nonviable. Affected fetuses typically present with fetal hydrops, short-limbed dwarfism, and a marked disorganization of chondro-osseous calcification and may present with polydactyly and additional nonskeletal malformations.
    Defects in LBR may be a cause of Reynolds syndrome (REYNS) [MIM:613471]. It is a syndrome specifically associating limited cutaneous systemic sclerosis and primary biliray cirrhosis. It is characterized by liver disease, telangiectasia, abrupt onset of digital paleness or cyanosis in response to cold exposure or stress (Raynaud phenomenon), and variable features of scleroderma. The liver disease is characterized by pruritis, jaundice, hepatomegaly, increased serum alkaline phosphatase and positive serum mitochondrial autoantibodies, all consistent with primary biliary cirrhosis.
  • Sequence similarities
    Belongs to the ERG4/ERG24 family.
  • Post-translational
    modifications
    Phosphorylated by CDK1 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures. Phosphorylation of LBR and HP1 proteins may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle.
  • Cellular localization
    Nucleus inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DHCR 14B antibody
    • DHCR14B antibody
    • Integral nuclear envelope inner membrane protein antibody
    • Lamin-B receptor antibody
    • LBR antibody
    • LBR_HUMAN antibody
    • LMN 2R antibody
    • LMN2R antibody
    • MGC9041 antibody
    • PHA antibody
    • PRO0650 antibody
    see all

Images

  • Anti-Lamin B Receptor antibody (ab169306) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 71 kDa

  • All lanes : Anti-Lamin B Receptor antibody (ab169306) at 1 µg/ml

    Lane 1 : Lamin B Receptor transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Developed using the ECL technique.

    Predicted band size: 71 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling Lamin B Receptor with ab169306 at 3µg/ml.
  • Immunofluorescent analysis of HeLa cells labeling Lamin B Receptor with ab169306 at 10µg/ml.

References

This product has been referenced in:
  • Jeong K  et al. Insulin-response epigenetic activation of Egr-1 and JunB genes at the nuclear periphery by A-type lamin-associated pY19-Caveolin-2 in the inner nuclear membrane. Nucleic Acids Res 43:3114-27 (2015). Rat . Read more (PubMed: 25753664) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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