Key features and details
- Mouse polyclonal to Lamin B Receptor/LBR
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Lamin B Receptor/LBR antibody
See all Lamin B Receptor/LBR primary antibodies
DescriptionMouse polyclonal to Lamin B Receptor/LBR
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Full length protein corresponding to Human Lamin B Receptor/LBR aa 1-615.
MPSRKFADGEVVRGRWPGSSLYYEVEILSHDSTSQLYTVKYKDGTELELK ENDIKPLTSFRQRKGGSTSSSPSRRRGSRSRSRSRSPGRPPKSARRSASA SHQADIKEARREVEVKLTPLILKPFGNSISRYNGEPEHIERNDAPHKNTQ EKFSLSQESSYIATQYSLRPRREEVKLKEIDSKEEKYVAKELAVRTFEVT PIRAKDLEFGGVPGVFLIMFGLPVFLFLLLLMCKQKDPSLLNFPPPLPAL YELWETRVFGVYLLWFLIQVLFYLLPIGKVVEGTPLIDGRRLKYRLNGFY AFILTSAVIGTSLFQGVEFHYVYSHFLQFALAATVFCVVLSVYLYMRSLK APRNDLSPASSGNAVYDFFIGRELNPRIGTFDLKYFCELRPGLIGWVVIN LVMLLAEMKIQDRAVPSLAMILVNSFQLLYVVDALWNEEALLTTMDIIHD GFGFMLAFGDLVWVPFIYSFQAFYLVSHPNEVSWPMASLIIVLKLCGYVI FRGANSQKNAFRKNPSDPKLAHLKTIHTSTGKNLLVSGWWGFVRHPNYLG DLIMALAWSLPCGFNHILPYFYIIYFTMLLVHREARDEYHCKKKYGVAWE KYCQRVPYRIFPYIY
Database link: NP_002287.2
- Lamin B Receptor/LBR transfected 293T cell lysate; Jurkat cell lysate; Human small intestine tissue; HeLa cells.
This product was previously labelled as Lamin B Receptor
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.4
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab169306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa.|
|IHC-P||Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionAnchors the lamina and the heterochromatin to the inner nuclear membrane.
Involvement in diseaseDefects in LBR are a cause of Pelger-Huet anomaly (PHA) [MIM:169400]. PHA is an autosomal dominant inherited abnormality of neutrophils, characterized by reduced nuclear segmentation and an apparently looser chromatin structure. Heterozygotes show hypolobulated neutrophil nuclei with coarse chromatin. Presumed homozygous individuals have ovoid neutrophil nuclei, as well as varying degrees of developmental delay, epilepsy, and skeletal abnormalities.
Defects in LBR are the cause of hydrops-ectopic calcification-moth-eaten skeletal dysplasia (HEM) [MIM:215140]; also known as Greenberg skeletal dysplasia. HEM is a rare autosomal recessive chondrodystrophy characterized by early in utero lethality and, therefore, considered to be nonviable. Affected fetuses typically present with fetal hydrops, short-limbed dwarfism, and a marked disorganization of chondro-osseous calcification and may present with polydactyly and additional nonskeletal malformations.
Defects in LBR may be a cause of Reynolds syndrome (REYNS) [MIM:613471]. It is a syndrome specifically associating limited cutaneous systemic sclerosis and primary biliray cirrhosis. It is characterized by liver disease, telangiectasia, abrupt onset of digital paleness or cyanosis in response to cold exposure or stress (Raynaud phenomenon), and variable features of scleroderma. The liver disease is characterized by pruritis, jaundice, hepatomegaly, increased serum alkaline phosphatase and positive serum mitochondrial autoantibodies, all consistent with primary biliary cirrhosis.
Sequence similaritiesBelongs to the ERG4/ERG24 family.
modificationsPhosphorylated by CDK1 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures. Phosphorylation of LBR and HP1 proteins may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle.
Cellular localizationNucleus inner membrane.
- Information by UniProt
- DHCR 14B antibody
- DHCR14B antibody
- Integral nuclear envelope inner membrane protein antibody
Anti-Lamin B Receptor/LBR antibody (ab169306) at 1 µg/ml + Jurkat cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 71 kDa
All lanes : Anti-Lamin B Receptor/LBR antibody (ab169306) at 1 µg/ml
Lane 1 : Lamin B Receptor/LBR transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Developed using the ECL technique.
Predicted band size: 71 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling Lamin B Receptor/LBR with ab169306 at 3µg/ml.
Immunofluorescent analysis of HeLa cells labeling Lamin B Receptor/LBR with ab169306 at 10µg/ml.
ab169306 has been referenced in 2 publications.
- Chen K et al. Protective effect of histone methyltransferase NSD3 on ISO-induced cardiac hypertrophy. FEBS Lett 593:2556-2565 (2019). PubMed: 31254363
- Jeong K et al. Insulin-response epigenetic activation of Egr-1 and JunB genes at the nuclear periphery by A-type lamin-associated pY19-Caveolin-2 in the inner nuclear membrane. Nucleic Acids Res 43:3114-27 (2015). Rat . PubMed: 25753664