Key features and details
- Rabbit polyclonal to Lamin B1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Lamin B1 antibody
See all Lamin B1 primary antibodies
DescriptionRabbit polyclonal to Lamin B1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment (His-tag) corresponding to Human Lamin B1 aa 52-233. (Expressed in E.coli).
SLETENSALQLQVTEREEVRGRELTGLKALYETELADARRALDDTARERA KLQIELGKCKAEHDQLLLNYAKKESDLNGAQIKLREYEAALNSKDAALAT ALGDKKSLEGDLEDLKDQIAQLEASLAAAKKQLADETLLKVDLENRCQSL TEDLEFRKSMYEEEINETRRKHETRLVEVDSG
Database link: P20700
- IHC-P: Human brain tissue. WB: Recombinant human Lamin B1 protein; HeLa cell lysate; Human lung lysate, Pig brain and kidney lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231282 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 66 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionLamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Involvement in diseaseDefects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
Sequence similaritiesBelongs to the intermediate filament family.
modificationsB-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Cellular localizationNucleus inner membrane.
- Information by UniProt
- ADLD antibody
- lamin B1 antibody
- Lamin-B1 antibody
All lanes : Anti-Lamin B1 antibody (ab231282) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Human lung lysate
Lane 3 : Pig brain lysate
Lane 4 : Pig kidney lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 66 kDa
Anti-Lamin B1 antibody (ab231282) at 2 µg/ml + Recombinant human Lamin B1 protein.
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 66 kDa
Paraffin-embedded human brain tissue stained for Lamin B1 using ab231282 at 20 μg/ml in immunohistochemical analysis. DAb staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231282 has not yet been referenced specifically in any publications.