Overview

  • Product name

  • Description

    Rabbit polyclonal to Lamin B1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-P, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Lamin B1 aa 570-586 (C terminal).
    Sequence:

    FHQQGTPRASNRSCAIM


    Database link: P20700

  • Positive control

    • WB: Rat brain tissue lysate IHC-P:Mammary cancer sections; IHC-F: Rat Intestine Tissue ICC: A549 Cells

Properties

Applications

Our Abpromise guarantee covers the use of ab65986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 0.5 - 1 µg/ml. Formalin or acetone fixed tissues.
WB Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use a concentration of 0.5 - 1 µg/ml. Acetone fixed.

Target

  • Function

    Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • Involvement in disease

    Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • Cellular localization

    Nucleus inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADLD antibody
    • lamin B1 antibody
    • Lamin-B1 antibody
    • LMN antibody
    • LMN2 antibody
    • LMNB antibody
    • Lmnb1 antibody
    • LMNB1_HUMAN antibody
    • MGC111419 antibody
    • OTTHUMP00000159218 antibody
    see all

Images

  • All lanes : Anti-Lamin B1 antibody (ab65986)

    Lane 1 : MCF-7 Cell lysate
    Lane 2 : HeLa Cell lysate

    Predicted band size: 66 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human Mammary Cancer Tissue staining Lamin B1 with ab65986 
  • Immunohistochemistry (Frozen sections) of Rat Intestine Tissue staining Lamin B1 with ab65986 

  • Immunocytochemistry of A549 Cells staining Lamin B1 with ab65986.

  • Immunohistochemical analysis of paraffin-embedded mammary cancer tissue staining Lamin B1 in cytoplasm using ab65986 at 1µg/ml. DAB chromogenic reaction.

  • Anti-Lamin B1 antibody (ab65986) at 1 µg/ml + rat brain tissue lysate

    Predicted band size: 66 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Chen T  et al. PAK4 phosphorylates fumarase and blocks TGF-ß-induced cell growth arrest in lung cancer cells. Cancer Res N/A:N/A (2019). Read more (PubMed: 30683654) »
  • Lin YC  et al. Nifedipine Modulates Renal Lipogenesis via the AMPK-SREBP Transcriptional Pathway. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30934807) »
See all 9 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (umbilical endothelial artery cells)
Permeabilization
Yes - 1% Triton
Specification
umbilical endothelial artery cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Inês Tomé

Verified customer

Submitted Apr 19 2019

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