Immunocytochemistry/ Immunofluorescence abreview for Anti-Lamin B1 antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (umbilical endothelial artery cells)
Permeabilization
Yes - 1% Triton
Specification
umbilical endothelial artery cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Diluent: BSA 1%
Concentration
1 µg/ml

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L) Antibo
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
Staining also worked using Methanol (5 min, dry air) as a fixation method.

Inês Tomé

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Submitted Apr 19 2019

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