Key features and details
- Rabbit polyclonal to Lamin B1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Lamin B1 antibody
See all Lamin B1 primary antibodies
DescriptionRabbit polyclonal to Lamin B1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Lamin B1 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P20700
- Jurkat cells. Human breast carcinoma.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use for 30 minutes at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 66 kDa.|
FunctionLamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Involvement in diseaseDefects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
Sequence similaritiesBelongs to the intermediate filament family.
modificationsB-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Cellular localizationNucleus inner membrane.
- Information by UniProt
- ADLD antibody
- lamin B1 antibody
- Lamin-B1 antibody
ab74393, at 1/25 dilution, staining Lamin B1 in formalin-fixed, paraffin-embedded human breast carcinoma tissue sections by Immunohistochemistry.
Anti-Lamin B1 antibody (ab74393) at 1/25 dilution + lysate prepared from HepG2 cells
Predicted band size: 66 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab74393 has not yet been referenced specifically in any publications.