Overview

  • Product name

  • Description

    Chicken polyclonal to Lamin B1
  • Host species

    Chicken
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human Lamin B1 aa 400-500 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab16375)

  • Positive control

    • Recombinant Human Lamin B1 protein (ab114163) can be used as a positive control in WB. This antibody gave a positive signal in the following human whole cell lysates: HeLa; Jurkat; A431; NIH3T3.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab90169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 66 kDa).

Target

  • Function

    Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • Involvement in disease

    Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • Cellular localization

    Nucleus inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADLD antibody
    • lamin B1 antibody
    • Lamin-B1 antibody
    • LMN antibody
    • LMN2 antibody
    • LMNB antibody
    • Lmnb1 antibody
    • LMNB1_HUMAN antibody
    • MGC111419 antibody
    • OTTHUMP00000159218 antibody
    see all

Images

  • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Empty
    Lane 3: LMNB1 HAP1 knockout whole cell lysate (20 µg)
    Lane 4: Empty
    Lanes 1 - 4: Merged signal (red and green). Green - ab90169 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab90169 was shown to specifically react with Lamin B1 in wild-type HAP1 cells. No bands were observed when Lamin B1 knockout samples were used. Wild-type and Lamin B1 knockout samples were subjected to SDS-PAGE.  ab90169 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Donkey anti-Chicken IgG H&L (IRDye® 800CW) and Donkey anti Mouse IgG H&L secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Anti-Lamin B1 antibody (ab90169) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 18 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes
  • All lanes : Anti-Lamin B1 antibody (ab90169) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Chicken IgY - H&L (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 73 kDa why is the actual band size different from the predicted?
    Additional bands at: 28 kDa, 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes

References

ab90169 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (Xenopus S3 cells)
Permeabilization
Yes - 0.25% Triton X-100
Specification
Xenopus S3 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Sep 11 2018

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