For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    lamin-b1-antibody-epr22165-121-ab229025.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Apoptosis Nucleus Lamins
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Lamin B1 antibody [EPR22165-121] (ab229025)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (5) Submit a question References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
  • Multiplex immunohistochemistry - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
  • Western blot - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
  • Western blot - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
  • Western blot - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
  • Immunoprecipitation - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
  • Immunoprecipitation - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
  • Flow Cytometry (Intracellular) - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
  • Flow Cytometry (Intracellular) - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
  • Anti-Lamin B1 antibody [EPR22165-121] (ab229025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22165-121] to Lamin B1
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP, mIHC
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
Product image
Anti-GUCY1B3 antibody [EPR8822] (ab154841)
Dye
Product image
Mitochondrial Staining Kit - NIR Fluorescence - Cytopainter (ab176747)
Primary
Product image
Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)

View more associated products

Overview

  • Product name

    Anti-Lamin B1 antibody [EPR22165-121]
    See all Lamin B1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22165-121] to Lamin B1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP, mIHCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HAP1, HepG2, Caco-2, RAW 264.7, PC-12 and NIH/3T3 whole cell lysate; Human lymph node tissue; Mouse brain and heart tissue lysate; Rat brain, heart and spleen tissue lysate. IHC-P: Human breast and liver tissue; Mouse liver tissue, Rat cerebrum tissue. mIHC: Human liver tissue. ICC/IF: HeLa, HAP1, and NIH/3T3 cells. Flow: HeLa, HAP1 and NIH/3T3 cells. IP: NIH/3T3 and HeLa whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22165-121
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Lamins
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Envelope
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class V
    • Lamins
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Lamins

Associated products

  • Alternative Versions

    • Anti-Lamin B1 antibody [EPR22165-121] - BSA and Azide free (ab239399)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human LMNB1 (Lamin B1) knockout HeLa cell line (ab255404)
  • KO cell lysates

    • Human LMNB1 (Lamin B1) knockout HeLa cell lysate (ab263825)
  • Related Products

    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab229025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.
WB (5)
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

ICC/IF
1/1000.
IP
1/30.
mIHC
1/4000.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

ICC/IF
1/1000.
IP
1/30.
mIHC
1/4000.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • Involvement in disease

    Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • Cellular localization

    Nucleus inner membrane.
  • Target information above from: UniProt accession P20700 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4001 Human
    • Entrez Gene: 16906 Mouse
    • Entrez Gene: 116685 Rat
    • Omim: 150340 Human
    • SwissProt: P20700 Human
    • SwissProt: P14733 Mouse
    • SwissProt: P70615 Rat
    • Unigene: 89497 Human
    • Unigene: 4105 Mouse
    • Unigene: 11362 Rat
    see all
  • Alternative names

    • ADLD antibody
    • lamin B1 antibody
    • Lamin-B1 antibody
    • LMN antibody
    • LMN2 antibody
    • LMNB antibody
    • Lmnb1 antibody
    • LMNB1_HUMAN antibody
    • MGC111419 antibody
    • OTTHUMP00000159218 antibody
    see all

Images

  • Western blot - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    Western blot - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    All lanes : Anti-Lamin B1 antibody [EPR22165-121] (ab229025) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : LMNB1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 66-70 kDa why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab229025 observed at 66-70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab229025 was shown to react with Lamin B1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255404 (knockout cell lysate ab263825) was used. Wild-type HeLa and LMNB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab229025 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Multiplex immunohistochemistry - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    Multiplex immunohistochemistry - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)

    Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue.

    Panel A:  Merged staining of Collagen VI (ab182744; green), anti-CD68 (ab213363; red) and anti-Lamin B1 (ab229025; magenta).

    Panel B: Anti-Collagen VI (green) stained on extracellular matrix.

    Panel C: Anti-CD68 (red) stained on Kupffer cells.

    Panel D: Anti-Lamin B1 (magenta) stained on nuclear envelope.

    Key protocol steps:  The section was incubated in three rounds of staining with ab182744 (1/1000 dilution), ab213363 (1/1000 dilution) and ab229025 (1/4000 dilution) for 30 mins at room temperature. Each round was followed by tyramide signal amplification with the appropriate fluorophore. Heat mediated antigen retrieval was used (Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins after every round of antibody/fluorophore staining.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    DAPI was used as a nuclear counter stain. A ready-to-use anti-Rabbit and Mouse Polymer HRP was used as a secondary.

  • Western blot - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    Western blot - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    All lanes : Anti-Lamin B1 antibody [EPR22165-121] (ab229025) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : Lamin B1 knockout HAP1 whole cell lysate
    Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 66 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    ab229025 was shown to specifically react with Lamin B1 in wild-type HAP1 cells as signal was lost in Lamin B1 knockout cells. Wild-type and Lamin B1 knockout samples were subjected to SDS-PAGE. ab229025 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Lamin B1 (Green) with ab229025 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing nuclear membranous staining in HeLa cells. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as a counterstain at 1/200 dilution. The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Alexa Fluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue stained for Lamin B1 using ab229025 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear envelope staining on mouse liver (PMID: 19925772) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) cells labeling Lamin B1 (Green) with ab229025 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing nuclear membranous staining in NIH/3T3 cells. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as a counterstain at 1/200 dilution. The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Alexa Fluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

     

  • Western blot - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    Western blot - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    All lanes : Anti-Lamin B1 antibody [EPR22165-121] (ab229025) at 1/1000 dilution

    Lane 1 : Human lymph node tissue lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 4 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 5 : Mouse brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution
    Lanes 2-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 66 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time:

    Lane 1:3 seconds;

    Lanes 2,3,4 and 5:10 seconds.

  • Western blot - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    Western blot - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    All lanes : Anti-Lamin B1 antibody [EPR22165-121] (ab229025) at 1/1000 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat heart tissue lysate
    Lane 4 : Rat spleen tissue lysate
    Lane 5 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 7 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 66 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time:

    Lane 1:10 seconds; Lanes 2 and 3: 59 seconds; Lane 4:3 seconds; Lanes 5,6 and 7:6 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue stained for Lamin B1 using ab229025 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear envelope staining on rat cerebrum (PMID: 26469707, PMID: 19925772) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)

    Immunohistochemical analysis of paraffin-embedded human breast tissue stained for Lamin B1 using ab229025 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear envelope staining on human breast (PMID: 26469707) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    Immunoprecipitation - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)

    Lamin B1 was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab229025 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229025 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (input).
    Lane 2: ab229025 IP in HeLa whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229025 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 seconds.

    A very weak band around 66 kDa detected in lane 3 is due to spill over from lane2 (+).

  • Immunoprecipitation - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    Immunoprecipitation - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)

    Lamin B1 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab229025 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229025 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10 μg (input).
    Lane 2: ab229025 IP in NIH/3T3 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229025 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 seconds.

  • Flow Cytometry (Intracellular) - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    Flow Cytometry (Intracellular) - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cell line labeling Lamin B1 with ab229025 at 1/500 dilution (Red) compared with aRabbit monoclonal IgGisotype control (ab172730) (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor� 488, ab150077) was used as the secondary antibody at 1/2000 dilution. 

     

     

  • Flow Cytometry (Intracellular) - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    Flow Cytometry (Intracellular) - Anti-Lamin B1 antibody [EPR22165-121] (ab229025)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Lamin B1 with ab229025 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgGisotype control (ab172730) (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor� 488, ab150077) was used as the secondary antibody at 1/2000 dilution. 

     

     

  • Anti-Lamin B1 antibody [EPR22165-121] (ab229025)
    Anti-Lamin B1 antibody [EPR22165-121] (ab229025)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (3)

Publishing research using ab229025? Please let us know so that we can cite the reference in this datasheet.

ab229025 has been referenced in 3 publications.

  • Tang D  et al. Silencing LMNB1 Contributes to the Suppression of Lung Adenocarcinoma Development. Cancer Manag Res 13:2633-2642 (2021). PubMed: 33776481
  • Chen J  et al. ACSL4 promotes hepatocellular carcinoma progression via c-Myc stability mediated by ERK/FBW7/c-Myc axis. Oncogenesis 9:42 (2020). PubMed: 32350243
  • Roos NJ  et al. Lapatinib Activates the Kelch-Like ECH-Associated Protein 1-Nuclear Factor Erythroid 2-Related Factor 2 Pathway in HepG2 Cells. Front Pharmacol 11:944 (2020). PubMed: 32694997

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-Lamin B1 antibody [EPR22165-121]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (3611-RF cells)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
25 µg
Specification
3611-RF cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 04 2022

Western blot abreview for Anti-Lamin B1 antibody [EPR22165-121]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEFs)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
25 µg
Specification
MEFs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 04 2022

Western blot abreview for Anti-Lamin B1 antibody [EPR22165-121]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Monkey Cell lysate - whole cell (COS-1 cells)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
25 µg
Specification
COS-1 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 27 2022

Western blot abreview for Anti-Lamin B1 antibody [EPR22165-121]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Dog Cell lysate - whole cell (MDCK cells)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
25 µg
Specification
MDCK cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 27 2022

Western blot abreview for Anti-Lamin B1 antibody [EPR22165-121]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
25 µg
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 27 2022

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.