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Cell Biology Apoptosis Nucleus Lamins
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Validated using a knockout cell line

Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)

  • Datasheet
  • SDS
Reviews (96)Q&A (16)References (625)

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Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
  • Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
  • Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
  • Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
  • Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)

Key features and details

  • Rabbit polyclonal to Lamin B1 - Nuclear Envelope Marker
  • Suitable for: ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Lamin B1 antibody - Nuclear Envelope Marker
    See all Lamin B1 primary antibodies
  • Description

    Rabbit polyclonal to Lamin B1 - Nuclear Envelope Marker
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Mouse Lamin B1 aa 400-500 (internal sequence) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab16375)

  • General notes

    Lamin B1 and Lamin B antibodies are extremely useful as nuclear loading controls for use with nuclear extracts. When using Lamin B1 antibodies as nuclear loading controls, be aware that in apoptotic cells Lamin B1 is cleaved (Kottke TJ et al.). Lamin B1 will also be removed from a nuclear prep if the nuclear membranes are spun out. This antibody was designed to be a nuclear loading control however it has not yet been tested in appropriate lysates.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Lamin B1 and Lamin B antibodies are extremely useful as nuclear loading controls for use with nuclear extracts. When using Lamin B1 antibodies as nuclear loading controls, be aware that in apoptotic cells Lamin B1 is cleaved (Kottke TJ et al.). Lamin B1 will also be removed from a nuclear prep if the nuclear membranes are spun out. This antibody was designed to be a nuclear loading control however it has not yet been tested in appropriate lysates.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Lamins
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Envelope
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class V
    • Lamins
    • Isotype/Loading Controls
    • Loading Controls
    • Lamin B1 - Nuclear
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Lamins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Immunizing Peptide (Blocking)

    • Mouse Lamin B1 peptide (ab16375)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Mouse
Human
IHC-P
Human
WB
Human
All applications
Chicken
Pig
Xenopus laevis
Indian muntjac
Zebrafish
Application Abreviews Notes
ICC/IF (24)
Use a concentration of 0.1 - 1 µg/ml.
WB (58)
Use a concentration of 0.1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 66 kDa).

We recommend Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773).

IHC-P (5)
Use a concentration of 0.1 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
ICC/IF
Use a concentration of 0.1 - 1 µg/ml.
WB
Use a concentration of 0.1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 66 kDa).

We recommend Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773).

IHC-P
Use a concentration of 0.1 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • Involvement in disease

    Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • Cellular localization

    Nucleus inner membrane.
  • Target information above from: UniProt accession P20700 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 396223 Chicken
    • Entrez Gene: 4001 Human
    • Entrez Gene: 16906 Mouse
    • Entrez Gene: 100513342 Pig
    • Entrez Gene: 116685 Rat
    • Entrez Gene: 379745 Xenopus laevis
    • Entrez Gene: 195816 Zebrafish
    • Omim: 150340 Human
    • SwissProt: P14731 Chicken
    • SwissProt: P20700 Human
    • SwissProt: P14733 Mouse
    • SwissProt: P70615 Rat
    • Unigene: 89497 Human
    • Unigene: 4105 Mouse
    • Unigene: 11362 Rat
    see all
  • Alternative names

    • ADLD antibody
    • lamin B1 antibody
    • Lamin-B1 antibody
    • LMN antibody
    • LMN2 antibody
    • LMNB antibody
    • Lmnb1 antibody
    • LMNB1_HUMAN antibody
    • MGC111419 antibody
    • OTTHUMP00000159218 antibody
    see all

Images

  • Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
    Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
    All lanes : Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : PC12 whole cell lysate
    Lane 3 : NIH 3T3 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Predicted band size: 66 kDa



    Blocking buffer: 3% Milk

    Gel type: MOPS

    Exposure Time: 2 minutes

    Observed band size: 73 kDa

    Additional bands: 46 kDa

  • Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
    Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: empty lane
    Lane 3: KO HAP1 LMNB1 whole cell lysate (20 µg)
    Lane 4: empty lane
    Lanes 1 - 4: Merged signal (red and green). Green - ab16048 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab16048 was shown to specifically react with  LMNB1 (Lamin B1) in wild type HAP1 cells. No band was observed when LMNB1 (Lamin B1) knockout samples were used. Ab16048 LMNB1 (Lamin B1) and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 0.1 μg per mL and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
    Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)Image courtesy of Marilena Ciciarello and Patrizia Lavia, University 'La Sapienza' CNR, Italy

    Human and mouse cells stained with ab16048 (1/500). The cells were fixed and permeabilized in 4% formaldehyde, 0.2% Tritonm X100 for 10 minutes at room temperature, then washed 3x in PBS.

    A: HeLa cells + ab16048 (green)
    B: HeLa cells counterstained with DAPI (blue)
    C: 3T3 cells + ab16048 (green)
    D: 3T3 cells counterstained with DAPI (blue)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)

    IHC image of Lamin B1 staining in Human normal Liver formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16048, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
    Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)

    Lane 1: Wild-type HAP1 nuclear lysate (10 µg)
    Lane 2: Lamin B1 knockout HAP1 nuclear lysate (10 µg)

    Lanes 1 and 2: Green signal from target - ab16048 observed at 68 kDa. Red signal from loading control - ab10799 observed at 18 kDa.

    ab16048 was shown to specifically react with lamin B1 in wild-type HAP1 cells. No band was observed knockout samples were used. Wild-type and lamin B1 knockout samples were subjected to SDS-PAGE. ab16048 and ab10799 (loading control to histone H3 at 0.1µg/mL) were both incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
    Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
    All lanes : Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1/1000 dilution

    Lane 1 : Hela whole cell lysate
    Lane 2 : Hela whole cell lysate with Mouse Lamin B1 peptide (ab16375) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Alexa fluor goat polyclonal to Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 68-70 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
    Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)

    ICC/IF image of ab16048 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16048, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
    Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)This image is courtesy of an anonymous Abreview
    Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1/1000 dilution + Human Pancreatic cell line - whole cell lysate at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit antibody at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 68 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)This image is courtesy of an anonymous Abreview

    ab16048 staining Lamin B1 in human infantile fibromatosis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% FBS/BSA for 3 hours at room temperature; antigen retrieval was by heat mediation in Tris pH9. Samples were incubated with primary antibody (1/100 in TBS + 1% BSA + 1% FBS) for 16 hours. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
    Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)Image courtesy of Marilena Ciciarello and Patrizia Lavia, University 'La Sapienza' CNR, Italy

    Human and mouse cells stained with ab16048 (1/500). The cells were fixed in 100% methanol for 6 minutes at -20°C, then washed once in PBS.

    A: HeLa cells + ab16048 (green)
    B: HeLa cells counterstained with DAPI (blue)
    C: 3T3 cells + ab16048 (green)
    D: 3T3 cells counterstained with DAPI (blue)

     

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (625)

Publishing research using ab16048? Please let us know so that we can cite the reference in this datasheet.

ab16048 has been referenced in 625 publications.

  • Godavarthy PS  et al. The vascular bone marrow niche influences outcome in chronic myeloid leukemia via the E-selectin - SCL/TAL1 - CD44 axis. Haematologica 105:136-147 (2020). PubMed: 31018977
  • Spadotto V  et al. PRMT1-mediated methylation of the microprocessor-associated proteins regulates microRNA biogenesis. Nucleic Acids Res 48:96-115 (2020). PubMed: 31777917
  • Xie S  et al. Andrographolide Protects Against Adverse Cardiac Remodeling After Myocardial Infarction through Enhancing Nrf2 Signaling Pathway. Int J Biol Sci 16:12-26 (2020). PubMed: 31892842
  • Lowe DJ  et al. Chronic irradiation of human cells reduces histone levels and deregulates gene expression. Sci Rep 10:2200 (2020). PubMed: 32042076
  • Wintner O  et al. A Unified Linear Viscoelastic Model of the Cell Nucleus Defines the Mechanical Contributions of Lamins and Chromatin. Adv Sci (Weinh) 7:1901222 (2020). PubMed: 32328409
View all Publications for this product

Customer reviews and Q&As

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1-10 of 112 Abreviews or Q&A

Western blot abreview for Anti-Lamin B1 antibody - Nuclear Envelope Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human coronary artery endothelial cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Treatment
10Gy of X-ray, collected 7 days after irradiation
Specification
Human coronary artery endothelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Read More

Sylwia Kabacik

Verified customer

Submitted Dec 17 2020

Western blot abreview for Anti-Lamin B1 antibody - Nuclear Envelope Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - nuclear (Gastric Cancer Cell Line)
Gel Running Conditions
Reduced Denaturing
Loading amount
3 µg
Specification
Gastric Cancer Cell Line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Oct 29 2020

Western blot abreview for Anti-Lamin B1 antibody - Nuclear Envelope Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - nuclear (RAW264.7)
Gel Running Conditions
Reduced Denaturing
Loading amount
3 µg
Specification
RAW264.7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Oct 29 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Lamin B1 antibody - Nuclear Envelope Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (primary adult microvascular endothelial cells)
Permeabilization
Yes - 0.1% triton X
Specification
primary adult microvascular endothelial cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Sep 10 2020

Western blot abreview for Anti-Lamin B1 antibody - Nuclear Envelope Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - nuclear (soleus muscle)
Gel Running Conditions
Reduced Non-Denaturing (Native) (10)
Loading amount
10 µg
Specification
soleus muscle
Blocking step
No blocking step used for 12 hour(s) and 0 minute(s) · Concentration: 1/1000µg/mL · Temperature: 4°C
Read More

Inna Paramonova

Verified customer

Submitted Sep 26 2019

Western blot abreview for Anti-Lamin B1 antibody - Nuclear Envelope Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (melanoma)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
melanoma
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jun 26 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-Lamin B1 antibody - Nuclear Envelope Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Melanoma)
Permeabilization
Yes - 0.2% Triton X100
Specification
Human Melanoma
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Apr 30 2019

Western blot abreview for Anti-Lamin B1 antibody - Nuclear Envelope Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - nuclear (MEF)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
10 µg
Specification
MEF
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jan 09 2019

Western blot abreview for Anti-Lamin B1 antibody - Nuclear Envelope Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - nuclear (B Cells)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
30 µg
Specification
B Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Dec 18 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Lamin B1 antibody - Nuclear Envelope Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa Cells)
Permeabilization
Yes - Triton 0.3%
Specification
HeLa Cells
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Nov 13 2018

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