• Product name

  • Description

    Rabbit polyclonal to Lamin B2
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Xenopus laevis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 221-470 of Human Lamin B2 (NP_116126)

  • Positive control

    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cells IHC-P: Cal27 xenograft



Our Abpromise guarantee covers the use of ab97513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 22341447
WB 1/500 - 1/3000. Predicted molecular weight: 68 kDa.
IHC-P 1/100 - 1/250.


  • Function

    Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • Involvement in disease

    Defects in LMNB2 are a cause of partial acquired lipodystrophy (APLD) [MIM:608709]. A rare childhood disease characterized by loss of subcutaneous fat from the face and trunk. Fat deposition on the pelvic girdle and lower limbs is normal or excessive. Most frequently, onset between 5 and 15 years of age. Most affected subjects are females and some show no other abnormality, but many develop glomerulonephritis, diabetes mellitus, hyperlipidemia, and complement deficiency. Mental retardation in some cases. APLD is a sporadic disorder of unknown etiology.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational

    B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • Cellular localization

    Nucleus inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • LAMB 2 antibody
    • LAMB2 antibody
    • Lamin-B2 antibody
    • LMN 2 antibody
    • LMN B2 antibody
    • LMN2 antibody
    • LMNB 2 antibody
    • LMNB2 antibody
    • LMNB2_HUMAN antibody
    • MGC2721 antibody
    • RGD1563803 antibody
    see all


  • All lanes : Anti-Lamin B2 antibody (ab97513) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 68 kDa

    7.5% SDS PAGE
  • Immunohistochemical analysis of Lamin B2 in paraffin embedded Cal27 Xenograft, using ab97513 at a 1/100 dilution.


This product has been referenced in:

  • Cioni JM  et al. Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons. Cell 176:56-72.e15 (2019). Read more (PubMed: 30612743) »
  • Yoon BC  et al. Local translation of extranuclear lamin B promotes axon maintenance. Cell 148:752-64 (2012). ICC/IF ; Xenopus laevis . Read more (PubMed: 22341447) »
See all 2 Publications for this product

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