Product nameAnti-Lamin B2 antibody
See all Lamin B2 primary antibodies
DescriptionRabbit polyclonal to Lamin B2
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human, Xenopus laevis
Recombinant fragment, corresponding to a region within amino acids 221-470 of Human Lamin B2 (NP_116126)
- WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cells IHC-P: Cal27 xenograft
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 22341447|
|WB||1/500 - 1/3000. Predicted molecular weight: 68 kDa.|
|IHC-P||1/100 - 1/250.|
FunctionLamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Involvement in diseaseDefects in LMNB2 are a cause of partial acquired lipodystrophy (APLD) [MIM:608709]. A rare childhood disease characterized by loss of subcutaneous fat from the face and trunk. Fat deposition on the pelvic girdle and lower limbs is normal or excessive. Most frequently, onset between 5 and 15 years of age. Most affected subjects are females and some show no other abnormality, but many develop glomerulonephritis, diabetes mellitus, hyperlipidemia, and complement deficiency. Mental retardation in some cases. APLD is a sporadic disorder of unknown etiology.
Sequence similaritiesBelongs to the intermediate filament family.
modificationsB-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Cellular localizationNucleus inner membrane.
- Information by UniProt
- LAMB 2 antibody
- LAMB2 antibody
- Lamin-B2 antibody
All lanes : Anti-Lamin B2 antibody (ab97513) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa
7.5% SDS PAGE
Immunohistochemical analysis of Lamin B2 in paraffin embedded Cal27 Xenograft, using ab97513 at a 1/100 dilution.
This product has been referenced in:
- Cioni JM et al. Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons. Cell 176:56-72.e15 (2019). Read more (PubMed: 30612743) »
- Yoon BC et al. Local translation of extranuclear lamin B promotes axon maintenance. Cell 148:752-64 (2012). ICC/IF ; Xenopus laevis . Read more (PubMed: 22341447) »