Recombinant
RabMAb

Recombinant Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)

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Overview

  • Product name

    Anti-Lamin B2 antibody [EPR9700(B)]
    See all Lamin B2 primary antibodies

  • Description

    Rabbit monoclonal [EPR9700(B)] to Lamin B2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Lamin B2 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HepG2, Jurkat, HeLa, and Raji cell lysates. HeLa cells.

  • General notes

    A trial size is available to purchase for this antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab138516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    • Function

      Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

    • Involvement in disease

      Defects in LMNB2 are a cause of partial acquired lipodystrophy (APLD) [MIM:608709]. A rare childhood disease characterized by loss of subcutaneous fat from the face and trunk. Fat deposition on the pelvic girdle and lower limbs is normal or excessive. Most frequently, onset between 5 and 15 years of age. Most affected subjects are females and some show no other abnormality, but many develop glomerulonephritis, diabetes mellitus, hyperlipidemia, and complement deficiency. Mental retardation in some cases. APLD is a sporadic disorder of unknown etiology.

    • Sequence similarities

      Belongs to the intermediate filament family.

    • Post-translational
      modifications

      B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.

    • Cellular localization

      Nucleus inner membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • LAMB 2 antibody
        • LAMB2 antibody
        • Lamin-B2 antibody
        • LMN 2 antibody
        • LMN B2 antibody
        • LMN2 antibody
        • LMNB 2 antibody
        • LMNB2 antibody
        • LMNB2_HUMAN antibody
        • MGC2721 antibody
        • RGD1563803 antibody
        see all

      Images

      • Western blot - Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)
        Western blot - Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)
        All lanes : Anti-Lamin B2 antibody [EPR9700(B)] (ab138516) at 1/1000 dilution

        Lane 1 : HepG2 cell lysate
        Lane 2 : Jurkat cell lysate
        Lane 3 : HeLa cell lysate
        Lane 4 : Raji cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 68 kDa
        Observed band size: 68 kDa

      • Flow Cytometry - Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)
        Flow Cytometry - Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)
        Flow Cytometrical analysis of permeabilized HeLa cells with ab138516 at a dilution of 1/10 (red) or a rabbit IgG negative (green).

      References

      This product has been referenced in:

      • Hesketh EL  et al. Transient association of MCM complex proteins with the nuclear matrix during initiation of mammalian DNA replication. Cell Cycle 14:333-41 (2015). Read more (PubMed: 25659032) »
      See 1 Publication for this product

      Publishing research using ab138516? Please let us know so that we can cite the reference in this datasheet.

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