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    lamin-b2-antibody-epr9700b-ab138516.pdf

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Cell Biology Apoptosis Nucleus Lamins
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RecombinantRabMAb

Recombinant Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)

  • Datasheet
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Western blot - Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)
  • Flow Cytometry (Intracellular) - Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)
  • Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9700(B)] to Lamin B2
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Lamin B2 antibody [EPR9700(B)]
    See all Lamin B2 primary antibodies
  • Description

    Rabbit monoclonal [EPR9700(B)] to Lamin B2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Lamin B2 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HepG2, Jurkat, HeLa, and Raji cell lysates. HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9700(B)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Lamins
    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Scaffold Proteins
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nucleoplasm
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class V
    • Lamins
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Lamins

Associated products

  • Alternative Versions

    • Anti-Lamin B2 antibody [EPR9700(B)] - BSA and Azide free (ab248858)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab138516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Function

    Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • Involvement in disease

    Defects in LMNB2 are a cause of partial acquired lipodystrophy (APLD) [MIM:608709]. A rare childhood disease characterized by loss of subcutaneous fat from the face and trunk. Fat deposition on the pelvic girdle and lower limbs is normal or excessive. Most frequently, onset between 5 and 15 years of age. Most affected subjects are females and some show no other abnormality, but many develop glomerulonephritis, diabetes mellitus, hyperlipidemia, and complement deficiency. Mental retardation in some cases. APLD is a sporadic disorder of unknown etiology.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • Cellular localization

    Nucleus inner membrane.
  • Target information above from: UniProt accession Q03252 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 84823 Human
    • Entrez Gene: 16907 Mouse
    • Entrez Gene: 299625 Rat
    • Omim: 150341 Human
    • SwissProt: Q03252 Human
    • SwissProt: P21619 Mouse
    • Unigene: 728836 Human
    • Unigene: 7362 Mouse
    • Alternative names

      • LAMB 2 antibody
      • LAMB2 antibody
      • Lamin-B2 antibody
      • LMN 2 antibody
      • LMN B2 antibody
      • LMN2 antibody
      • LMNB 2 antibody
      • LMNB2 antibody
      • LMNB2_HUMAN antibody
      • MGC2721 antibody
      • RGD1563803 antibody
      see all

    Images

    • Western blot - Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)
      Western blot - Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)
      All lanes : Anti-Lamin B2 antibody [EPR9700(B)] (ab138516) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 68 kDa
      Observed band size: 68 kDa

    • Flow Cytometry (Intracellular) - Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)
      Flow Cytometry (Intracellular) - Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)

      Intracellular flow cytometrical analysis of permeabilized HeLa cells with ab138516 at a dilution of 1/10 (red) or a rabbit IgG negative (green).

       

    • Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)
      Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab138516? Please let us know so that we can cite the reference in this datasheet.

    ab138516 has been referenced in 1 publication.

    • Hesketh EL  et al. Transient association of MCM complex proteins with the nuclear matrix during initiation of mammalian DNA replication. Cell Cycle 14:333-41 (2015). PubMed: 25659032

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