Recombinant Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)
![Western blot - Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)](https://www.abcam.com/ps/products/138/ab138516/Images/ab138516-153815-anti-lamin-b2-antibody-epr9700b-western-blot.jpg "Western blot - Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9700(B)] to Lamin B2
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Lamin B2 antibody [EPR9700(B)]
See all Lamin B2 primary antibodies -
Description
Rabbit monoclonal [EPR9700(B)] to Lamin B2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Lamin B2 aa 50-150. The exact sequence is proprietary.
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Positive control
- HepG2, Jurkat, HeLa, and Raji cell lysates. HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9700(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab138516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.
Target
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Function
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. -
Involvement in disease
Defects in LMNB2 are a cause of partial acquired lipodystrophy (APLD) [MIM:608709]. A rare childhood disease characterized by loss of subcutaneous fat from the face and trunk. Fat deposition on the pelvic girdle and lower limbs is normal or excessive. Most frequently, onset between 5 and 15 years of age. Most affected subjects are females and some show no other abnormality, but many develop glomerulonephritis, diabetes mellitus, hyperlipidemia, and complement deficiency. Mental retardation in some cases. APLD is a sporadic disorder of unknown etiology. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsB-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. -
Cellular localization
Nucleus inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 84823 Human
- Entrez Gene: 16907 Mouse
- Entrez Gene: 299625 Rat
- Omim: 150341 Human
- SwissProt: Q03252 Human
- SwissProt: P21619 Mouse
- Unigene: 728836 Human
- Unigene: 7362 Mouse
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Alternative names
- LAMB 2 antibody
- LAMB2 antibody
- Lamin-B2 antibody
see all
Images
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Western blot - Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)All lanes : Anti-Lamin B2 antibody [EPR9700(B)] (ab138516) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa -
![Flow Cytometry (Intracellular) - Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)](https://www.abcam.com/ps/products/138/ab138516/Images/ab138516-153814-anti-lamin-b2-antibody-epr9700b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)Intracellular flow cytometrical analysis of permeabilized HeLa cells with ab138516 at a dilution of 1/10 (red) or a rabbit IgG negative (green).
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![Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)](https://www.abcam.com/ps/products/138/ab138516/Images/ab138516-3-benefits-of-recombinant-antibodies.png)
Anti-Lamin B2 antibody [EPR9700(B)] (ab138516)
Datasheets and documents
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Datasheet download
References (1)
ab138516 has been referenced in 1 publication.
- Hesketh EL et al. Transient association of MCM complex proteins with the nuclear matrix during initiation of mammalian DNA replication. Cell Cycle 14:333-41 (2015). PubMed: 25659032
