Name: Anti-Lamin B2 antibody [EPR9701(B)]
Brand: RabMAb
SKU: ab151735
Rating: 4 (1 reviews)

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Primary - Rabbit Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)
Flow Cyt, WB, IHC-P, ICC/IF, IP

Secondary - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC-P, WB, ELISA, IP

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Product name

Anti-Lamin B2 antibody [EPR9701(B)]
See all Lamin B2 primary antibodies
Description

Rabbit monoclonal [EPR9701(B)] to Lamin B2
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, WB, IHC-P, ICC/IF, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human Lamin B2 aa 550-650 (C terminal). The exact sequence is proprietary.
Positive control
- HepG2, Jurkat, HeLa, and Raji cell lysates; Human bladder carcinoma and colon tissues; HepG2 cells.
General notes

A trial size is available to purchase for this antibody.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9701(B)
Isotype

IgG
Research areas
- Cell Biology
- Apoptosis
- Nucleus
- Lamins
- Cancer
- Cell Death
- Apoptosis
- Nucleus
- Lamins

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls

Our Abpromise guarantee covers the use of ab151735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt		Use at an assay dependent concentration.
WB		1/1000 - 1/10000. Predicted molecular weight: 68 kDa.
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF		1/250 - 1/500.
IP		1/10 - 1/100.

Function

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Involvement in disease

Defects in LMNB2 are a cause of partial acquired lipodystrophy (APLD) [MIM:608709]. A rare childhood disease characterized by loss of subcutaneous fat from the face and trunk. Fat deposition on the pelvic girdle and lower limbs is normal or excessive. Most frequently, onset between 5 and 15 years of age. Most affected subjects are females and some show no other abnormality, but many develop glomerulonephritis, diabetes mellitus, hyperlipidemia, and complement deficiency. Mental retardation in some cases. APLD is a sporadic disorder of unknown etiology.
Sequence similarities

Belongs to the intermediate filament family.
Post-translational
modifications

B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Cellular localization

Nucleus inner membrane.
Target information above from: UniProt accession Q03252 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 84823 Human
- Entrez Gene: 16907 Mouse
- Entrez Gene: 299625 Rat
- Omim: 150341 Human
- SwissProt: Q03252 Human
- SwissProt: P21619 Mouse
- Unigene: 728836 Human
- Unigene: 7362 Mouse
Alternative names
- LAMB 2 antibody
- LAMB2 antibody
- Lamin-B2 antibody
- LMN 2 antibody
- LMN B2 antibody
- LMN2 antibody
- LMNB 2 antibody
- LMNB2 antibody
- LMNB2_HUMAN antibody
- MGC2721 antibody
- RGD1563803 antibody
see all

Western blot - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)

All lanes : Anti-Lamin B2 antibody [EPR9701(B)] (ab151735) at 1/1000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Raji cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 68 kDa
Flow Cytometry - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)

ab151735 staining Lamin B2 in the human cell line HepG2 (human hepatocellular carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control: Rabbit monoclonal IgG (Black)

Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)

Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labeling Lamin B2 with ab151735 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Lamin B2 with ab151735 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)

Immunofluorescent analysis of HepG2 cells labeling Lamin B2 with ab151735 at 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)

Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab151735 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)

Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using ab151735 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)

Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using ab151735 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

This product has been referenced in:

Pan X et al. Activation of Nrf2/HO-1 signal with Myricetin for attenuating ECM degradation in human chondrocytes and ameliorating the murine osteoarthritis. Int Immunopharmacol 75:105742 (2019). Read more (PubMed: 31325727) »
Bikkul MU et al. Telomere elongation through hTERT immortalization leads to chromosome repositioning in control cells and genomic instability in Hutchinson-Gilford progeria syndrome fibroblasts, expressing a novel SUN1 isoform. Genes Chromosomes Cancer 58:341-356 (2019). Read more (PubMed: 30474255) »

See all 13 Publications for this product

Publishing research using ab151735? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Application

Immunocytochemistry/ Immunofluorescence

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C

Sample

Mouse Cell (Neuronal progenitor cell line)

Specification

Neuronal progenitor cell line

Permeabilization

Yes - PBS 1% BSA 0,1% Saponine

Fixative

Paraformaldehyde

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Submitted Apr 03 2014

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Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Applications

Target

Function

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Lamin B2 antibody [EPR9701(B)]

Post-translational
modifications