- Datasheet
- Specific References (25)
- Protocols
Overview
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Product nameAnti-Lamin B2 antibody [LN43]
See all Lamin B2 primary antibodies -
DescriptionMouse monoclonal [LN43] to Lamin B2
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Host speciesMouse
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SpecificityThis antibody reacts with an epitope located in the C-terminal part of lamin B2. Lamins do not appear to be universally distributed among different cell and tissue types. Please see the listed positive controls for this antibody. Other cell/tissue types have not been tested.
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Tested applicationsSuitable for: ICC, Flow Cyt, IHC-Fr, WB, IP, ICC/IFmore details
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Species reactivityReacts with: Mouse, Hamster, Human, Xenopus laevis, Zebrafish
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Immunogen
Tissue, cells or virus corresponding to Lamin B2. Immunogen = detergent insoluble fraction of potoroo cell line PtK1.
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EpitopeEpitope located in the C-terminal part of lamin B2.
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Positive control
- IHC-Fr: testicular germ cell tumour sections (see Machiels et al 1997), parabasal cells of skin epidermis (see Broers et al). WB: NCI-H125 nuclear cell lysate Jurkat cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberLN43
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab8983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | 1/400. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr | 1/1. (when used with an avidin-biotinylated HRP complex as detection agent). |
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| WB | 1/100 - 1/1000. Detects a band of approximately 65-67 kDa. | |
| IP | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 22403714 |
Target
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FunctionLamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
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Involvement in diseaseDefects in LMNB2 are a cause of partial acquired lipodystrophy (APLD) [MIM:608709]. A rare childhood disease characterized by loss of subcutaneous fat from the face and trunk. Fat deposition on the pelvic girdle and lower limbs is normal or excessive. Most frequently, onset between 5 and 15 years of age. Most affected subjects are females and some show no other abnormality, but many develop glomerulonephritis, diabetes mellitus, hyperlipidemia, and complement deficiency. Mental retardation in some cases. APLD is a sporadic disorder of unknown etiology.
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Sequence similaritiesBelongs to the intermediate filament family.
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Post-translational
modificationsB-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. -
Cellular localizationNucleus inner membrane.
- Information by UniProt
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Database links
- Entrez Gene: 84823 Human
- Entrez Gene: 16907 Mouse
- Omim: 150341 Human
- SwissProt: Q03252 Human
- SwissProt: P21619 Mouse
- Unigene: 728836 Human
- Unigene: 7362 Mouse
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Alternative names
- LAMB 2 antibody
- LAMB2 antibody
- Lamin-B2 antibody
see all
Images
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![Immunofluorescence - Anti-Lamin B2 antibody [LN43] (ab8983)](https://www.abcam.com/ps/products/8/ab8983/Images/ab8983-218487-ab8983.jpg)
Immunofluorescence - Anti-Lamin B2 antibody [LN43] (ab8983)IF staining of a 9 days old zebrafish embryo.
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![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody [LN43] (ab8983)](https://www.abcam.com/ps/products/8/ab8983/Images/Lamin-B2-Primary-antibodies-ab8983-12.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody [LN43] (ab8983)Image from Tsai YJ et al., PLoS One. 2012;7(3):e32820. Epub 2012 Mar 5. Fig 5.; doi:10.1371/journal.pone.0032820; March 5, 2012, PLoS ONE 7(3): e32820.Immunofluorescence analysis of HeLa cells in telophase, staining Lamin B2 with ab8983. -
![Western blot - Anti-Lamin B2 antibody [LN43] (ab8983)](https://www.abcam.com/ps/products/8/ab8983/Images/Lamin-B2-Primary-antibodies-ab8983-5.jpg)
Western blot - Anti-Lamin B2 antibody [LN43] (ab8983)All lanes : Anti-Lamin B2 antibody [LN43] (ab8983) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 4 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 75 kDa (why is the actual band size different from the predicted?)
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
![Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (ab8983)](https://www.abcam.com/ps/products/8/ab8983/Images/ab8983-218486-ab8983.jpg)
Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (ab8983)IHC-Fr of human kidney showing nuclear lamina staining in the ductal epithelium.
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![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody [LN43] (ab8983)](https://www.abcam.com/ps/products/8/ab8983/Images/ab8983_4a.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody [LN43] (ab8983)This image is courtesy of Marilena Ciciarello & Patrizia Lavia, University La SapienzaHeLa cells were fixed in 100% methanol for 6 minutes at -20°C then stained with ab8983 (1/400). This image shows that ab8983 clearly stains the nuclear envelope (green). The DNA is stained with DAPI (blue).
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![Immunoprecipitation - Anti-Lamin B2 antibody [LN43] (ab8983)](https://www.abcam.com/ps/products/8/ab8983/Images/Lamin-B2-Primary-antibodies-ab8983-11.jpg)
Immunoprecipitation - Anti-Lamin B2 antibody [LN43] (ab8983)Lamin B2 - Nuclear Envelope Marker was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to Lamin B2 - Nuclear Envelope Marker (ab8983) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab8983.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 73kDa: Lamin B2 -
![Flow Cytometry - Anti-Lamin B2 antibody [LN43] (ab8983)](https://www.abcam.com/ps/products/8/ab8983/Images/Lamin-B2-Primary-antibodies-ab8983-8.jpg)
Flow Cytometry - Anti-Lamin B2 antibody [LN43] (ab8983)Overlay histogram showing HeLA cells stained with ab8983 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8983, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References
This product has been referenced in:
- Han KY et al. SIDR: simultaneous isolation and parallel sequencing of genomic DNA and total RNA from single cells. Genome Res 28:75-87 (2018). Read more (PubMed: 29208629) »
- Ma Y et al. Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis. Oncotarget 8:104813-104830 (2017). Read more (PubMed: 29285216) »
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