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Tissue, cells or virus corresponding to Lamin B2. Immunogen = detergent insoluble fraction of potoroo cell line PtK1.
Our Abpromise guarantee covers the use of ab8983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
(when used with an avidin-biotinylated HRP complex as detection agent).
|WB||1/100 - 1/1000. Detects a band of approximately 65-67 kDa.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22403714|
IF staining of a 9 days old zebrafish embryo.
IHC-Fr of human kidney showing nuclear lamina staining in the ductal epithelium.
HeLa cells were fixed in 100% methanol for 6 minutes at -20°C then stained with ab8983 (1/400). This image shows that ab8983 clearly stains the nuclear envelope (green). The DNA is stained with DAPI (blue).
Overlay histogram showing HeLA cells stained with ab8983 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8983, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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