Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)

Rat monoclonal Laminin 2 alpha antibody [4H8-2]. Validated in WB, IP, ELISA, IHC, ICC, ICC/IF and tested in Mouse, Human. Cited in 23 publication(s). Independently reviewed in 6 review(s).

Overview

  • Product name

    Anti-Laminin 2 alpha antibody [4H8-2]
    See all Laminin 2 alpha primary antibodies
  • Description

    Rat monoclonal [4H8-2] to Laminin 2 alpha
  • Host species

    Rat
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, WB, ELISA, ICC, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Heart Laminin 2 (Mouse).

  • Epitope

    The epitope recognized by the antibody resides in the N-terminal portion of the alpha 2 chain of laminin.
  • Positive control

    • Human tongue.
  • General notes

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab11576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 4 - 8 µg/ml.

This concentration was determined for acetone-fixed frozen sections of human tongue.

ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 400 kDa.
ELISA Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function

    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificity

    Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone.
  • Involvement in disease

    Defects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI.
  • Sequence similarities

    Contains 17 laminin EGF-like domains.
    Contains 5 laminin G-like domains.
    Contains 2 laminin IV type A domains.
    Contains 1 laminin N-terminal domain.
  • Domain

    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI, IV and G are globular.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane. Major component.
  • Information by UniProt
  • Database links

  • Alternative names

    • LAMA 2 antibody
    • LAMA2 antibody
    • LAMA2_HUMAN antibody
    • Laminin alpha 2 (merosin congenital muscular dystrophy) antibody
    • Laminin alpha 2 antibody
    • Laminin alpha 2 chain antibody
    • Laminin alpha 2 subunit antibody
    • Laminin M antibody
    • Laminin M chain antibody
    • Laminin subunit alpha-2 antibody
    • Laminin-12 subunit alpha antibody
    • Laminin-2 subunit alpha antibody
    • Laminin-4 subunit alpha antibody
    • LAMM antibody
    • Merosin heavy chain antibody
    see all

Images

  • ab11576 staining C57B6 mouse skeletal muscle cryostate tissue sections by IHC-Fr.  The section was acetone fixed and blocked with 5% BSA for 30 mintes at RT, prior to incubation with the primary antibody (diluted 1/200 in blocking buffer) for 2 hours.  A rhodamine-conjugated goat anti-rat antibody was used as the secondary.

    See Abreview

References

This product has been referenced in:

  • Aeffner F  et al. Validation of a Muscle-Specific Tissue Image Analysis Tool for Quantitative Assessment of Dystrophin Staining in Frozen Muscle Biopsies. Arch Pathol Lab Med 143:197-205 (2019). Read more (PubMed: 30168727) »
  • Benz F  et al. Low wnt/ß-catenin signaling determines leaky vessels in the subfornical organ and affects water homeostasis in mice. Elife 8:N/A (2019). Read more (PubMed: 30932814) »
See all 28 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole heart)
Gel Running Conditions
Reduced Denaturing (Gel 8%)
Loading amount
50 µg
Specification
Whole heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 11 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Methanol

Abcam user community

Verified customer

Submitted Jul 30 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Guinea pig Tissue sections (Skeletal muscle)
Permeabilization
No
Specification
Skeletal muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Nov 13 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - pH 9.0 Citrate Buffer Antigen Retrieval
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 07 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (muscle)
Permeabilization
Yes - PBS triton0.5%
Specification
muscle
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 14 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (C57B6 mouse skeletal muscle cryostate sections)
Specification
C57B6 mouse skeletal muscle cryostate sections
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 13 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up