Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)
Key features and details
- Rat monoclonal [4H8-2] to Laminin 2 alpha
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-Laminin 2 alpha antibody [4H8-2]
See all Laminin 2 alpha primary antibodies -
Description
Rat monoclonal [4H8-2] to Laminin 2 alpha -
Host species
Rat -
Tested applications
Suitable for: IHC-Frmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Human -
Immunogen
Full length protein corresponding to Mouse Laminin 2 alpha. Heart Laminin 2 (Mouse).
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Epitope
The epitope recognized by the antibody resides in the N-terminal portion of the alpha 2 chain of laminin. -
Positive control
- IHC-Fr: Mouse tongue tissue.
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General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4H8-2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr | (5) |
Use a concentration of 4 - 8 µg/ml.
This concentration was determined for acetone-fixed frozen sections of human tongue. |
Notes |
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IHC-Fr
Use a concentration of 4 - 8 µg/ml. This concentration was determined for acetone-fixed frozen sections of human tongue. |
Target
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Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
Tissue specificity
Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone. -
Involvement in disease
Defects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI. -
Sequence similarities
Contains 17 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 2 laminin IV type A domains.
Contains 1 laminin N-terminal domain. -
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI, IV and G are globular. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. Major component. - Information by UniProt
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Database links
- Entrez Gene: 3908 Human
- Entrez Gene: 16773 Mouse
- Omim: 156225 Human
- SwissProt: P24043 Human
- SwissProt: Q60675 Mouse
- Unigene: 200841 Human
- Unigene: 256087 Mouse
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Alternative names
- LAMA 2 antibody
- LAMA2 antibody
- LAMA2_HUMAN antibody
see all
Images
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Immunohistochemical analysis of acetone-fixed frozen section of mouse tongue tissue labeling Laminin 2 alpha using ab11576 at 8 μg/mL, followed by a monoclonal Anti-Rat Kappa & Lamda light chains-FITC congugate at 1/40 dilution (Green).
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Immunohistochemistry (Frozen sections) analysis of Mouse muscle tissue labeling Laminin 2 alpha with ab11576 at 1/200 dilution. Tissue fixed with paraformaldehyde and permeabilized with 0.3% Triton X-100 in PBS for 30 minutes. Tissue blocked with 10% goat serum for 1 hour at room temperature followed by incubation with the primary antibody in 2% goat serum, 0.2% triton X-100 in PBS overnight at 4°C. Goat anti-rat IgG (Alexa Fluor® 568) was used as the secondary antibody at 1/500 dilution (red).
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ab11576 staining C57B6 mouse skeletal muscle cryostate tissue sections by IHC-Fr. The section was acetone fixed and blocked with 5% BSA for 30 mintes at RT, prior to incubation with the primary antibody (diluted 1/200 in blocking buffer) for 2 hours. A rhodamine-conjugated goat anti-rat antibody was used as the secondary.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (61)
ab11576 has been referenced in 61 publications.
- Kandasamy P et al. Control of backbone chemistry and chirality boost oligonucleotide splice switching activity. Nucleic Acids Res 50:5443-5466 (2022). PubMed: 35061895
- Zeng W et al. CPEB1 directs muscle stem cell activation by reprogramming the translational landscape. Nat Commun 13:947 (2022). PubMed: 35177647
- Kim JH et al. Bap1/SMN axis in Dpp4+ skeletal muscle mesenchymal cells regulates the neuromuscular system. JCI Insight 7:N/A (2022). PubMed: 35603786
- Ang SJ et al. Muscle 4EBP1 activation modifies the structure and function of the neuromuscular junction in mice. Nat Commun 13:7792 (2022). PubMed: 36526657
- Wosczyna MN et al. Targeting microRNA-mediated gene repression limits adipogenic conversion of skeletal muscle mesenchymal stromal cells. Cell Stem Cell 28:1323-1334.e8 (2021). PubMed: 33945794