Key features and details
- Mouse monoclonal [CL3450] to Laminin 2 alpha
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-Laminin 2 alpha antibody [CL3450]
See all Laminin 2 alpha primary antibodies
DescriptionMouse monoclonal [CL3450] to Laminin 2 alpha
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
EpitopeBinds to an epitope located within the peptide sequence DKLKPIKELEDNLKK as determined by overlapping synthetic peptides.
- IHC-P: Human heart, placenta and cerebral cortex tissues; Rat heart muscle and stomach tissues; Mouse heart muscle tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein A purified
Purification notesPurified from TCS
Our Abpromise guarantee covers the use of ab236762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 344 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificityPlacenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone.
Involvement in diseaseDefects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI.
Sequence similaritiesContains 17 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 2 laminin IV type A domains.
Contains 1 laminin N-terminal domain.
DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI, IV and G are globular.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. Major component.
- Information by UniProt
- LAMA 2 antibody
- LAMA2 antibody
- LAMA2_HUMAN antibody
All lanes : Anti-Laminin 2 alpha antibody [CL3450] (ab236762) at 1 µg/ml
Lane 1 : Purified human recombinant Laminin-211
Lane 2 : Purified human recombinant Laminin-221
Lane 3 : Purified human recombinant Laminin-332
Lane 4 : Purified human recombinant Laminin-421
Lane 5 : Purified human recombinant Laminin-511
Lane 6 : Purified human recombinant Laminin-121
Predicted band size: 344 kDa
Paraffin-embedded human heart tissue stained for Laminin 2 alpha using ab236762 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human placenta tissue stained for Laminin 2 alpha using ab236762 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human cerebral cortex tissue stained for Laminin 2 alpha using ab236762 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded rat heart tissue stained for Laminin 2 alpha using ab236762 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded rat stomach tissue stained for Laminin 2 alpha using ab236762 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded mouse heart tissue stained for Laminin 2 alpha using ab236762 at 1/200 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236762 has not yet been referenced specifically in any publications.