Recombinant
RabMAb

Recombinant Anti-Laminin 2 alpha antibody [EPR21890] - BSA and Azide free (ab238458)

Overview

  • Product name

    Anti-Laminin 2 alpha antibody [EPR21890] - BSA and Azide free
    See all Laminin 2 alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR21890] to Laminin 2 alpha - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Laminin 2 alpha aa 2700-2800. The exact sequence is proprietary.
    Database link: P24043

  • Positive control

    • IHC-P: Human cardiac muscle tissue.
  • General notes

    Ab238458 is the carrier-free version of ab219409. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238458 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificity

    Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone.
  • Involvement in disease

    Defects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI.
  • Sequence similarities

    Contains 17 laminin EGF-like domains.
    Contains 5 laminin G-like domains.
    Contains 2 laminin IV type A domains.
    Contains 1 laminin N-terminal domain.
  • Domain

    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI, IV and G are globular.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane. Major component.
  • Information by UniProt
  • Database links

  • Alternative names

    • LAMA 2 antibody
    • LAMA2 antibody
    • LAMA2_HUMAN antibody
    • Laminin alpha 2 (merosin congenital muscular dystrophy) antibody
    • Laminin alpha 2 antibody
    • Laminin alpha 2 chain antibody
    • Laminin alpha 2 subunit antibody
    • Laminin M antibody
    • Laminin M chain antibody
    • Laminin subunit alpha-2 antibody
    • Laminin-12 subunit alpha antibody
    • Laminin-2 subunit alpha antibody
    • Laminin-4 subunit alpha antibody
    • LAMM antibody
    • Merosin heavy chain antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Laminin 2 alpha with ab219409 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on villous basal membrane of human placenta is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219409).

  • Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Laminin 2 alpha with ab219409 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on basal lamina of human skeletal muscle (PMID: 26355035). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219409).

  • Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Laminin 2 alpha with ab219409 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on basal lamina of human cardiac muscle (PMID: 10773239). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab219409).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab238458 has not yet been referenced specifically in any publications.

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