For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit monoclonal [EPR8266] to Laminin 5 alpha 3
ICC/IF more details Unsuitable for:
Flow Cyt,IHC-P or IP
Synthetic peptide, corresponding to residues in Human Laminin 5 alpha 3 (UniProt: Q16787).
HeLa, HepG2, A431, Human skin and SH-SY5Y lysates; HepG2 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20ºC.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 367 kDa.
1/100 - 1/250.
Is unsuitable for Flow Cyt,IHC-P or IP.
Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin 5 is an isoform composed of three distinct subunits, alpha 3, beta 3 and gamma 2, which are bound to each other in a cross-shaped molecule by disulphide bonds. It is a complex glycoprotein thought to be involved in cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes. It is also involved in signal transduction via tyrosine phosphorylation of pp125-FAK and p80, and differentiation of keratinocytes.
The laminin alpha 3 subunit is also thought to be a component of laminin 6 and laminin 7.
Epiligrin 170 kDa subunit antibody
Epiligrin subunit alpha antibody
Immunocytochemistry/ Immunofluorescence - Anti-Laminin 5 alpha 3 antibody [EPR8266] (ab151715)
Immunofluorescent analysis of HepG2 cells labeling Laminin 5 alpha 3 with ab151715 at 1/100 dilution.
Western blot - Anti-Laminin 5 alpha 3 antibody [EPR8266] (ab151715)
All lanes : Anti-Laminin 5 alpha 3 antibody [EPR8266] (ab151715) at 1/1000 dilution Lane 1 : HeLa cell lysate Lane 2 : HepG2 cell lysate Lane 3 : A431 cell lysate Lane 4 : Human skin lysate Lane 5 : SH-SY5Y cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat anti-rabbit HRP at 1/2000 dilution Predicted band size: 367 kDa
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"