Immunohistochemistry (Frozen sections) abreview for Anti-Laminin 5 [P3H9-2] antibody

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Blocking step
Blocking One­ as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Human Tissue sections (cultured skin (NHEK, NB1RGB))
Specification
cultured skin (NHEK, NB1RGB)
Permeabilization
Yes - 0.1% TritonX-100
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 10% Blocking One­ in PBS(-)
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor­ 555 rabbit anti-mouse IgG(H+L)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 555
Dilution
1/200

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Submitted Sep 30 2013

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