Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198)
Key features and details
- Mouse monoclonal [CL3183] to Laminin alpha 4/LAMA4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Laminin alpha 4/LAMA4 antibody [CL3183]
See all Laminin alpha 4/LAMA4 primary antibodies -
Description
Mouse monoclonal [CL3183] to Laminin alpha 4/LAMA4 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Laminin alpha 4/LAMA4 aa 650-850.
Database link: Q16363 -
Epitope
Binds to an epitope located within the peptide sequence MANNLTNWSQNLQHF as determined by overlapping synthetic peptides. -
Positive control
- WB: Human placenta lysate. Lam-411 and Lam-421 purified recombinant human proteins. IHC-P: Human colon, pancreas, placenta and prostate tissue. Rat heart muscle, kidney, epididymis, rectum and stomach tissue. Mouse epididymis and stomach tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
CL3183 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab242198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 202 kDa.
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IHC-P | (1) |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 202 kDa. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
Tissue specificity
In adult, strong expression in heart, lung, ovary small and large intestines, placenta, liver; weak or no expression in skeletal muscle, kidney, pancreas, testis, prostate, brain. High expression in fetal lung and kidney. Expression in fetal and newborn tissues is observed in certain mesenchymal cells in tissues such as smooth muscle and dermis. -
Involvement in disease
Cardiomyopathy, dilated 1JJ -
Sequence similarities
Contains 4 laminin EGF-like domains.
Contains 5 laminin G-like domains. -
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain G is globular. -
Cellular localization
Secreted, extracellular space, extracellular matrix, basement membrane. Major component. - Information by UniProt
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Database links
- Entrez Gene: 3910 Human
- Entrez Gene: 16775 Mouse
- Entrez Gene: 309816 Rat
- Omim: 600133 Human
- SwissProt: Q16363 Human
- SwissProt: P97927 Mouse
- Unigene: 654572 Human
- Unigene: 258065 Mouse
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Alternative names
- CMD1JJ antibody
- DKFZp686D23145 antibody
- LAMA3 antibody
see all
Images
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Formalin-fixed, paraffin-embedded rat epididymis tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded mouse epididymis tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded mouse stomach tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded rat rectum tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded rat kidney tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded rat heart muscle tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded rat stomach tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Negative control - Formalin-fixed, paraffin-embedded human lymph node tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human placenta tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human pancreas tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human prostate tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1:200 dilution in immunohistochemical analysis.
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Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198) at 1 µg/ml + Human placenta tissue lysate
Predicted band size: 202 kDa -
All lanes : Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198) at 1 µg/ml
Lane 1 : Recombinant human Laminin-411 protein
Lane 2 : Recombinant human Laminin-421 protein
Lane 3 : Recombinant human Laminin-511 protein
Lane 4 : Recombinant human Laminin-121 protein
Lane 5 : Recombinant human Laminin-221 protein
Lane 6 : Recombinant human Laminin-332 protein
Predicted band size: 202 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab242198 has been referenced in 2 publications.
- Zheng B et al. LAMA4 upregulation is associated with high liver metastasis potential and poor survival outcome of Pancreatic Cancer. Theranostics 10:10274-10289 (2020). PubMed: 32929348
- Sheikh BN et al. Systematic Identification of Cell-Cell Communication Networks in the Developing Brain. iScience 21:273-287 (2019). PubMed: 31677479