Key features and details
- Mouse monoclonal [3H2] to Laminin alpha 4/LAMA4
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Laminin alpha 4/LAMA4 antibody [3H2]
See all Laminin alpha 4/LAMA4 primary antibodies
DescriptionMouse monoclonal [3H2] to Laminin alpha 4/LAMA4
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Human Laminin alpha 4/LAMA4. Immunoaffinity-purified Laminin 411 (laminin-8 (alpha4beta1gamma1) from platelets.
Database link: Q16363
- Human platelet lysate. Human embryonic kidney preparation.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab205568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 10 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 202 kDa.
|IP||Use a concentration of 0.5 mg/ml.|
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificityIn adult, strong expression in heart, lung, ovary small and large intestines, placenta, liver; weak or no expression in skeletal muscle, kidney, pancreas, testis, prostate, brain. High expression in fetal lung and kidney. Expression in fetal and newborn tissues is observed in certain mesenchymal cells in tissues such as smooth muscle and dermis.
Involvement in diseaseCardiomyopathy, dilated 1JJ
Sequence similaritiesContains 4 laminin EGF-like domains.
Contains 5 laminin G-like domains.
DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain G is globular.
Cellular localizationSecreted, extracellular space, extracellular matrix, basement membrane. Major component.
- Information by UniProt
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All lanes : Anti-Laminin alpha 4/LAMA4 antibody [3H2] (ab205568) at 1 µg/ml
Lane 1 : Human platelet lysate; reducing conditions
Lane 2 : Human platelet lysate; non-reducing conditions
Predicted band size: 202 kDa
Immunohistochemical analysis of Human embryonic kidney preparation labeling Laminin alpha 4/LAMA4 with ab205568 at 10 µg/ml followed by a fluorescent probe.
ab205568 has been referenced in 1 publication.
- Li Y et al. MicroRNA-200b is downregulated and suppresses metastasis by targeting LAMA4 in renal cell carcinoma. EBioMedicine 44:439-451 (2019). PubMed: 31130475