Key features and details
- Mouse monoclonal [CL3185] to Laminin alpha 4/LAMA4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-Laminin alpha 4/LAMA4 antibody [CL3185]
See all Laminin alpha 4/LAMA4 primary antibodies
DescriptionMouse monoclonal [CL3185] to Laminin alpha 4/LAMA4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
EpitopeBinds to an epitope located within the peptide sequence GALARKSALKTRLSD as determined by overlapping synthetic peptides.
- WB: Recombinant human Laminin-411 and Laminin-421 proteins. IHC-P: Human rectum, placenta and prostate tissue; mouseheart muscle, stomach and epididymis tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab242359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 203 kDa.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificityIn adult, strong expression in heart, lung, ovary small and large intestines, placenta, liver; weak or no expression in skeletal muscle, kidney, pancreas, testis, prostate, brain. High expression in fetal lung and kidney. Expression in fetal and newborn tissues is observed in certain mesenchymal cells in tissues such as smooth muscle and dermis.
Involvement in diseaseCardiomyopathy, dilated 1JJ
Sequence similaritiesContains 4 laminin EGF-like domains.
Contains 5 laminin G-like domains.
DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain G is globular.
Cellular localizationSecreted, extracellular space, extracellular matrix, basement membrane. Major component.
- Information by UniProt
- CMD1JJ antibody
- DKFZp686D23145 antibody
- LAMA3 antibody
All lanes : Anti-Laminin alpha 4/LAMA4 antibody [CL3185] (ab242359) at 1 µg/ml
Lane 1 : Recombinant humanan Laminin-411 protein
Lane 2 : Recombinant human Laminin-421 protein
Lane 3 : Recombinant human Laminin-511 protein
Lane 4 : Recombinant human Laminin-121 protein
Lane 5 : Recombinant human Laminin-221 protein
Lane 6 : Recombinant human Laminin-332 protein
Predicted band size: 203 kDa
Paraffin-embedded human rectum tissue stained for Laminin alpha 4/LAMA4 with ab242359 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human placenta tissue stained for Laminin alpha 4/LAMA4 with ab242359 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human prostate tissue stained for Laminin alpha 4/LAMA4 with ab242359 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded mouse heart muscle tissue stained for Laminin alpha 4/LAMA4 with ab242359 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded mouse stomach tissue stained for Laminin alpha 4/LAMA4 with ab242359 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded mouse epididymis tissue stained for Laminin alpha 4/LAMA4 with ab242359 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human lymph node tissue stained for Laminin alpha 4/LAMA4 with ab242359 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab242359 has not yet been referenced specifically in any publications.