Anti-Laminin alpha 5/LAMA5 antibody [4B12] (ab77175)
Key features and details
- Mouse monoclonal [4B12] to Laminin alpha 5/LAMA5
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Laminin alpha 5/LAMA5 antibody [4B12]
See all Laminin alpha 5/LAMA5 primary antibodies -
Description
Mouse monoclonal [4B12] to Laminin alpha 5/LAMA5 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IFmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Laminin alpha 5/LAMA5.
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Positive control
- Human embryonic lung (alveolar epithelium). Human kidney.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
4B12 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
Tissue specificity
Expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver. -
Sequence similarities
Contains 22 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain. -
Domain
Domain G is globular and is part of the major cell-binding site located in the long arm of the laminin heterotrimer. -
Cellular localization
Secreted, extracellular space, extracellular matrix, basement membrane. Major component. - Information by UniProt
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Database links
- Entrez Gene: 3911 Human
- Omim: 601033 Human
- SwissProt: O15230 Human
- Unigene: 473256 Human
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Alternative names
- KIAA0533 antibody
- KIAA1907 antibody
- LAMA5 antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab77175 has been referenced in 9 publications.
- Li L et al. Ibrutinib reverses IL-6-induced osimertinib resistance through inhibition of Laminin α5/FAK signaling. Commun Biol 5:155 (2022). PubMed: 35197546
- Le-Bel G et al. Influence of the Postmortem/Storage Time of Human Corneas on the Properties of Cultured Limbal Epithelial Cells. Cells 11:N/A (2022). PubMed: 36078126
- Majmundar AJ et al. Recessive NOS1AP variants impair actin remodeling and cause glomerulopathy in humans and mice. Sci Adv 7:N/A (2021). PubMed: 33523862
- Tuffin J et al. GlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening. Commun Biol 4:1351 (2021). PubMed: 34857869
- Ma C et al. Differential Labeling of Glycoproteins with Alkynyl Fucose Analogs. Int J Mol Sci 21:N/A (2020). PubMed: 32825463
- Tsai S et al. Development of primary human pancreatic cancer organoids, matched stromal and immune cells and 3D tumor microenvironment models. BMC Cancer 18:335 (2018). ICC/IF . PubMed: 29587663
- Hale LJ et al. 3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening. Nat Commun 9:5167 (2018). PubMed: 30514835
- Huang L et al. Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell- and patient-derived tumor organoids. Nat Med 21:1364-71 (2015). PubMed: 26501191
- Shimshek DR et al. The HSP70 molecular chaperone is not beneficial in a mouse model of alpha-synucleinopathy. PLoS One 5:e10014 (2010). PubMed: 20368804