Overview

  • Product name
    Anti-Laminin alpha 5 antibody [4C7]
    See all Laminin alpha 5 primary antibodies
  • Description
    Mouse monoclonal [4C7] to Laminin alpha 5
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Full length native protein (purified) corresponding to Human Laminin alpha 5.
    Database link: O15230

  • Positive control
    • Skin
  • General notes

    This antibody reacts with the terminal globular domain of the A chain. The staining with this antibody is sensitive to fixation of paraffin tissues and use of proteolytic enzymatic treatment.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab210957 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab17107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/5 - 1/20. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance
    Laminins are a family of heterotrimeric extracellular glycoproteins consisting of alpha, beta, and gamma chains. Laminins are thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin alpha 5 (LAMA5) belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Laminin alpha 5 is the alpha chain in the Laminin 15 complex which is a heterotrimer composed of alpha 5/beta 2/gamma 3 which are bound to each other by disulfide bonds into a cross shaped molecule comprising one long and three short arms with globules at each end.
  • Cellular localization
    Secreted; basement membrane.
  • Database links
  • Alternative names
    • KIAA0533 antibody
    • KIAA1907 antibody
    • LAMA5 antibody
    • Laminin alpha 5 chain antibody
    • Laminin subunit alpha 5 antibody
    • Laminin subunit alpha-5 antibody
    • Laminin, alpha 5 antibody
    • Laminin-10 subunit alpha antibody
    • Laminin-11 subunit alpha antibody
    • Laminin-15 subunit alpha antibody
    • RP11-157P1.6 antibody
    see all

Images

  • ab17107 at 1/25 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with the antibody for 16 hours. An HRP conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human renal carcinoma tissue, staining Laminin alpha 5 with ab17107.

References

This product has been referenced in:
  • Mackey AL  et al. Sequenced response of extracellular matrix deadhesion and fibrotic regulators after muscle damage is involved in protection against future injury in human skeletal muscle. FASEB J 25:1943-59 (2011). IHC-Fr ; Human . Read more (PubMed: 21368102) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

The antibody ab5654 is not tested in IHC-P application so Abpromise guarantee will not apply to this product. This antibody was however tested in WB so the guarantee will apply to WB only.

I also have read the details you have kindly provided and have following further questions for better understanding of the problem;

- What is the purchase order number? The number NL/Abcam 2698(N) does not match to any info we have in system.
- Are all the three product failed?
- What were the storage conditions of products?
- Did you observe non specific staining, no staining or low signal?
- Could you provide the species and type of tissues sections? e.g. human lungs
- How the sections were fixed?
- What sort of antigen retrieval was used? e.g. microwave with citrate buffer pH 6.0.
- Have you tried any permeabilization step?
- Did you try blocking the tissue sections?
- What was the species and conjugate of secondary antibodies? Have you used these successfully before?
- Did you try any sort of troubleshooting?
- Could you provide the images?

In order to get the result soon please provide all the requested details .Any additional information will also be helpful.

I will look forward to hearing from you soon.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (colon)
Specification
colon
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen Unmasking Solution, Citric Acid Based (Vector Lab)
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jul 03 2007

Answer

Thank you for your email. Our source for this antibody has kindly provided me with the following information. In the study by Engvall, 1990, it is stated: "4C7, binds to the globular domain at the end of the long arm (Engvall et al., 1986). This domain is the C-terminal portion of the A chain and the 4C7 antibody could therefore be against the A chain." Engvall E et al. Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits. Cell Regul 1:731-40 (1990). PubMed: 2099832 If you have any additional questions, please contact us again.

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Answer

This is the only information available to us regarding the immunogen and epitope. Further details can be found in the following two publications: Engvall E et al. Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits. Cell Regul 1:731-40 (1990). PubMed: 2099832 Kleinman HK et al. Laminin receptors for neurite formation. Proc Natl Acad Sci U S A 85:1282-6 (1988). PubMed: 2963341 If you have any additional questions, please contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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