Key features and details
- Mouse monoclonal [4B12] to Laminin alpha 5/LAMA5
- Suitable for: ELISA, IHC-P, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Laminin alpha 5/LAMA5 antibody [4B12]
See all Laminin alpha 5/LAMA5 primary antibodies
DescriptionMouse monoclonal [4B12] to Laminin alpha 5/LAMA5
Tested applicationsSuitable for: ELISA, IHC-P, IP, ICC/IFmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Human Laminin alpha 5/LAMA5.
- Human embryonic lung (alveolar epithelium). Human kidney.
This product was previously labelled as Laminin alpha 5
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityIon Exchange Chromatography
Our Abpromise guarantee covers the use of ab77175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificityExpressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver.
Sequence similaritiesContains 22 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain.
DomainDomain G is globular and is part of the major cell-binding site located in the long arm of the laminin heterotrimer.
Cellular localizationSecreted, extracellular space, extracellular matrix, basement membrane. Major component.
- Information by UniProt
- KIAA0533 antibody
- KIAA1907 antibody
- LAMA5 antibody
ab77175 has been referenced in 4 publications.
- Tsai S et al. Development of primary human pancreatic cancer organoids, matched stromal and immune cells and 3D tumor microenvironment models. BMC Cancer 18:335 (2018). ICC/IF . PubMed: 29587663
- Hale LJ et al. 3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening. Nat Commun 9:5167 (2018). PubMed: 30514835
- Huang L et al. Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell- and patient-derived tumor organoids. Nat Med 21:1364-71 (2015). PubMed: 26501191
- Shimshek DR et al. The HSP70 molecular chaperone is not beneficial in a mouse model of alpha-synucleinopathy. PLoS One 5:e10014 (2010). PubMed: 20368804