Overview

  • Product name

    Anti-Laminin alpha 5/LAMA5 antibody [4B12]
    See all Laminin alpha 5/LAMA5 primary antibodies
  • Description

    Mouse monoclonal [4B12] to Laminin alpha 5/LAMA5
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Laminin alpha 5/LAMA5.

  • Positive control

    • Human embryonic lung (alveolar epithelium). Human kidney.
  • General notes

     This product was previously labelled as Laminin alpha 5

     

Properties

Applications

Our Abpromise guarantee covers the use of ab77175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IP 1/20.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
    • Tissue specificity

      Expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver.
    • Sequence similarities

      Contains 22 laminin EGF-like domains.
      Contains 5 laminin G-like domains.
      Contains 1 laminin IV type A domain.
      Contains 1 laminin N-terminal domain.
    • Domain

      Domain G is globular and is part of the major cell-binding site located in the long arm of the laminin heterotrimer.
    • Cellular localization

      Secreted, extracellular space, extracellular matrix, basement membrane. Major component.
    • Information by UniProt
    • Database links

    • Alternative names

      • KIAA0533 antibody
      • KIAA1907 antibody
      • LAMA5 antibody
      • LAMA5_HUMAN antibody
      • Laminin alpha 5 chain antibody
      • Laminin subunit alpha 5 antibody
      • Laminin subunit alpha-5 antibody
      • Laminin, alpha 5 antibody
      • Laminin-10 subunit alpha antibody
      • Laminin-11 subunit alpha antibody
      • Laminin-15 subunit alpha antibody
      • RP11-157P1.6 antibody
      see all

    Images

    • Immunofluorescence of (A) Human embryonic lung alveolae epithelium and (B) Human kidney labelling Lamin alpha 5 with ab77175.

    References

    This product has been referenced in:

    • Tsai S  et al. Development of primary human pancreatic cancer organoids, matched stromal and immune cells and 3D tumor microenvironment models. BMC Cancer 18:335 (2018). ICC/IF . Read more (PubMed: 29587663) »
    • Hale LJ  et al. 3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening. Nat Commun 9:5167 (2018). Read more (PubMed: 30514835) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (RhE)
    Permeabilization
    No
    Specification
    RhE
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 4°C
    Fixative
    Methanol

    Abcam user community

    Verified customer

    Submitted Jul 06 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up