Immunocytochemistry/ Immunofluorescence abreview for Anti-Laminin alpha 5 antibody [4B12]

Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (RhE)
Permeabilization
No
Specification
RhE
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 4°C
Fixative
Methanol

Other product details

Incubation time
30 minute(s) · Temperature: 4°C
Dilution
1/200

Secondary antibody

Dilution
1/100
Name
Non-Abcam antibody was used: Cy™2 AffiniPure Goat Anti-Mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy2 ®

Additional data

Additional Notes
Double marquage de la laminine 5 (ab77175) en immunofluorescence et au DAPI sur coupes de RhE préalablement congelés en OCT.

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Submitted Jul 06 2018

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