Overview

  • Product name

    Anti-Laminin alpha 5/LAMA5 antibody [CL3118]
    See all Laminin alpha 5/LAMA5 primary antibodies
  • Description

    Mouse monoclonal [CL3118] to Laminin alpha 5/LAMA5
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Laminin alpha 5/LAMA5 aa 2555-2564. The exact sequence is proprietary.
    Sequence:

    RQGLVDRAQQ


    Database link: O15230

  • Epitope

    ab210957 binds to an epitope located within the peptide sequence RQGLVDRAQQ (aa 2555 - 2564).
  • Positive control

    • Recombinant protein fragment of human Laminin alpha 5/LAMA5; human placenta tissue lysate; human kidney, colon, heart, placenta, stomach, fallopian tube, and cervix tissues.
  • General notes

     This product was previously labelled as Laminin alpha 5

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    CL3118
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab210957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 400 kDa.

Target

  • Function

    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificity

    Expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver.
  • Sequence similarities

    Contains 22 laminin EGF-like domains.
    Contains 5 laminin G-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.
  • Domain

    Domain G is globular and is part of the major cell-binding site located in the long arm of the laminin heterotrimer.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix, basement membrane. Major component.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0533 antibody
    • KIAA1907 antibody
    • LAMA5 antibody
    • LAMA5_HUMAN antibody
    • Laminin alpha 5 chain antibody
    • Laminin subunit alpha 5 antibody
    • Laminin subunit alpha-5 antibody
    • Laminin, alpha 5 antibody
    • Laminin-10 subunit alpha antibody
    • Laminin-11 subunit alpha antibody
    • Laminin-15 subunit alpha antibody
    • RP11-157P1.6 antibody
    see all

Images

  • All lanes : Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (ab210957) at 1/500 dilution

    Lane 1 : Recombinant protein fragment of Human Laminin 511
    Lane 2 : Recombinant protein fragment of Human Laminin 521
    Lane 3 : Recombinant protein fragment of Human Laminin 121
    Lane 4 : Recombinant protein fragment of Human Laminin 221
    Lane 5 : Recombinant protein fragment of Human Laminin 332
    Lane 6 : Recombinant protein fragment of Human Laminin 411

    Developed using the ECL technique.

    Predicted band size: 400 kDa

  • Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (ab210957) at 1/500 dilution + Human placenta tissue lysate

    Developed using the ECL technique.

    Predicted band size: 400 kDa

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human heart tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human placenta tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human stomach tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human fallopian tube tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human cervix tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human lymph nodes tissue (negative control), labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

References

ab210957 has not yet been referenced specifically in any publications.

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