Recombinant
RabMAb

Recombinant Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)

Overview

  • Product name

    Anti-Laminin alpha 5/LAMA5 antibody [EPR18919]
    See all Laminin alpha 5/LAMA5 primary antibodies
  • Description

    Rabbit monoclonal [EPR18919] to Laminin alpha 5/LAMA5
  • Host species

    Rabbit
  • Specificity

    This antibody reacts weakly with Laminin alpha 3
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Mouse Laminin alpha 5/LAMA5 aa 1-350. The exact sequence is proprietary.
    Database link: Q61001

  • Positive control

    • WB: Mouse Laminin alpha 5/LAMA5 and alpha 3 fragment recombinant proteins; Human fetal kidney lysate; A549 whole cell lysate; Mouse P5 fetal kidney and P3 fetal lung lysates; Rat kidney lysate.
  • General notes

     This product was previously labelled as Laminin alpha 5

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18919
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 404 kDa (predicted molecular weight: 404 kDa).

Target

  • Function

    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificity

    Expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver.
  • Sequence similarities

    Contains 22 laminin EGF-like domains.
    Contains 5 laminin G-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.
  • Domain

    Domain G is globular and is part of the major cell-binding site located in the long arm of the laminin heterotrimer.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix, basement membrane. Major component.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0533 antibody
    • KIAA1907 antibody
    • LAMA5 antibody
    • LAMA5_HUMAN antibody
    • Laminin alpha 5 chain antibody
    • Laminin subunit alpha 5 antibody
    • Laminin subunit alpha-5 antibody
    • Laminin, alpha 5 antibody
    • Laminin-10 subunit alpha antibody
    • Laminin-11 subunit alpha antibody
    • Laminin-15 subunit alpha antibody
    • RP11-157P1.6 antibody
    see all

Images

  • All lanes : Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution

    Lane 1 : Mouse Laminin alpha 5/LAMA5 fragment recombinant protein
    Lane 2 : Mouse Laminin alpha 3 fragment recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 404 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2: 3 minutes.

    Mouse Laminin alpha 5/LAMA5 fragment recombinant protein contains aa46-304 with a His-Tag; Mouse Laminin alpha 3 fragment recombinant protein contains aa43-295 with a His-Tag.

  • All lanes : Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 404 kDa
    Observed band size: 404 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID:12559092; PMID:9624186).

  • All lanes : Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution

    Lane 1 : Mouse P5 fetal kidney lysate
    Lane 2 : Mouse P3 fetal lung lysate
    Lane 3 : Mouse liver lysate
    Lane 4 : Rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 404 kDa
    Observed band size: 404 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 and 2: 30 seconds; Lane 3 and 4: 3 minutes.

    The expression profile observed is consistent with what has been described in the literature (PMID:12559092; PMID:9624186).

     Negative control: mouse liver (PMID:7499364).

References

This product has been referenced in:

  • Zhang XM  et al. Down-Regulation of Laminin (LN)- a5 is Associated with Preeclampsia and Impairs Trophoblast Cell Viability and Invasiveness Through PI3K Signaling Pathway. Cell Physiol Biochem 51:2030-2040 (2018). Read more (PubMed: 30522103) »
See 1 Publication for this product

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