Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18919] to Laminin alpha 5/LAMA5 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Laminin alpha 5/LAMA5 antibody [EPR18919] - BSA and Azide free
See all Laminin alpha 5/LAMA5 primary antibodies
DescriptionRabbit monoclonal [EPR18919] to Laminin alpha 5/LAMA5 - BSA and Azide free
This antibody reacts weakly with Laminin alpha 3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Mouse Laminin alpha 5/LAMA5 aa 1-350. The exact sequence is proprietary.
Database link: Q61001
- WB: Mouse Laminin alpha 5/LAMA5 and alpha 3 fragment recombinant proteins; Human fetal kidney lysate; A549 whole cell lysate; Mouse P5 fetal kidney and P3 fetal lung lysates; Rat kidney lysate.
This product was previously labelled as Laminin alpha 5
This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This antibody is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab271947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 404 kDa (predicted molecular weight: 400 kDa).|
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificityExpressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver.
Sequence similaritiesContains 22 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain.
DomainDomain G is globular and is part of the major cell-binding site located in the long arm of the laminin heterotrimer.
Cellular localizationSecreted, extracellular space, extracellular matrix, basement membrane. Major component.
- Information by UniProt
- KIAA0533 antibody
- KIAA1907 antibody
- LAMA5 antibody
All lanes : Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 400 kDa
Observed band size: 404 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID:12559092; PMID:9624186).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, sodium azide and BSA (ab184330).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271947 has not yet been referenced specifically in any publications.